B cell phenotype and serum levels of interferons, BAFF, and APRIL in multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C)
Background Multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C) is a late complication of pediatric COVID-19, which follows weeks after the original SARS-CoV-2 infection, regardless of its severity. It is characterized by hyperinflammation, neutrophilia, lymphopenia, and activation of T cells with elevated IFN-γ. Observing the production of autoantibodies and parallels with systemic autoimmune disorders, such as systemic lupus erythematodes (SLE), we explored B cell phenotype and serum levels of type I, II, and III interferons, as well as the cytokines BAFF and APRIL in a cohort of MIS-C patients and healthy children after COVID-19. Results We documented a significant elevation of IFN-γ, but not IFN-α and IFN-λ in MIS-C patients. BAFF was elevated in MIS-C patient sera and accompanied by decreased BAFFR expression on all B cell subtypes. The proportion of plasmablasts was significantly lower in patients compared to healthy post-COVID children. We noted the pre-IVIG presence of ENA Ro60 autoantibodies in 4/35 tested MIS-C patients. Conclusions Our work shows the involvement of humoral immunity in MIS-C and hints at parallels with the pathophysiology of SLE, with autoreactive B cells driven towards autoantibody production by elevated BAFF. Graphical Abstract.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
|---|---|
| Enthalten in: |
Molecular and Cellular Pediatrics - 10(2023), 1 vom: 28. Okt. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Klocperk, Adam [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| Anmerkungen: |
© The Author(s) 2023 |
|---|
| doi: |
10.1186/s40348-023-00169-z |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR053558251 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR053558251 | ||
| 003 | DE-627 | ||
| 005 | 20231028064722.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231028s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s40348-023-00169-z |2 doi | |
| 035 | |a (DE-627)SPR053558251 | ||
| 035 | |a (SPR)s40348-023-00169-z-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Klocperk, Adam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a B cell phenotype and serum levels of interferons, BAFF, and APRIL in multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C) |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s) 2023 | ||
| 520 | |a Background Multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C) is a late complication of pediatric COVID-19, which follows weeks after the original SARS-CoV-2 infection, regardless of its severity. It is characterized by hyperinflammation, neutrophilia, lymphopenia, and activation of T cells with elevated IFN-γ. Observing the production of autoantibodies and parallels with systemic autoimmune disorders, such as systemic lupus erythematodes (SLE), we explored B cell phenotype and serum levels of type I, II, and III interferons, as well as the cytokines BAFF and APRIL in a cohort of MIS-C patients and healthy children after COVID-19. Results We documented a significant elevation of IFN-γ, but not IFN-α and IFN-λ in MIS-C patients. BAFF was elevated in MIS-C patient sera and accompanied by decreased BAFFR expression on all B cell subtypes. The proportion of plasmablasts was significantly lower in patients compared to healthy post-COVID children. We noted the pre-IVIG presence of ENA Ro60 autoantibodies in 4/35 tested MIS-C patients. Conclusions Our work shows the involvement of humoral immunity in MIS-C and hints at parallels with the pathophysiology of SLE, with autoreactive B cells driven towards autoantibody production by elevated BAFF. Graphical Abstract | ||
| 650 | 4 | |a PIMS-TS |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MIS-C |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a COVID-19 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Interferon |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a BAFF |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a APRIL |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a SLE |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Bloomfield, Marketa |4 aut | |
| 700 | 1 | |a Parackova, Zuzana |4 aut | |
| 700 | 1 | |a Aillot, Ludovic |4 aut | |
| 700 | 1 | |a Fremuth, Jiri |4 aut | |
| 700 | 1 | |a Sasek, Lumir |4 aut | |
| 700 | 1 | |a David, Jan |4 aut | |
| 700 | 1 | |a Fencl, Filip |4 aut | |
| 700 | 1 | |a Skotnicova, Aneta |4 aut | |
| 700 | 1 | |a Rejlova, Katerina |4 aut | |
| 700 | 1 | |a Magner, Martin |4 aut | |
| 700 | 1 | |a Hrusak, Ondrej |4 aut | |
| 700 | 1 | |a Sediva, Anna |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular and Cellular Pediatrics |d Berlin : SpringerOpen, 2014 |g 10(2023), 1 vom: 28. Okt. |w (DE-627)SPR036356883 |w (DE-600)2785551-X |x 2194-7791 |7 nnns |
| 773 | 1 | 8 | |g volume:10 |g year:2023 |g number:1 |g day:28 |g month:10 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1186/s40348-023-00169-z |z kostenfrei |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 10 |j 2023 |e 1 |b 28 |c 10 | ||