Evaluation of the effect of process parameters on the protein content of silk fibroin
Abstract Silk fibroin is a natural, protein-based biopolymer produced by silkworm and obtained from silkworm cocoons. It has such features as biocompatibility, biodegradability, high mechanical resistance, low immunogenicity and low inflammatory response. Owing to these properties, it attracts attention in various fields including tissue engineering and drug delivery. The silk fibroin production method basically consists of processes such as degumming, extraction, dialysis, centrifugation, and, if necessary, lyophilization. Since the production of silk fibroin from cocoons includes many steps, there are various parameters that affect the protein content of silk fibroin. In this study, it was aimed to validate the production process steps which mainly affect the silk fibroin content and to determine optimum parameters to obtain silk fibroin with higher protein content. For this purpose, the effects of the type of extraction agent (Ajisawa’s reagent or lithium bromide), the molecular weight cutoff value (MWCO) of the dialysis membrane, the dialysis time, and the centrifugation process were evaluated statistically with one-way analysis of variance method. According to the results, the highest protein content is obtained using, LiBr as extraction agent, 3500 Da as MWCO value of dialysis membrane, 2 days as dialysis time and 532 RCF for 5 min as conditions of centrifugation process..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:80 |
|---|---|
| Enthalten in: |
Polymer bulletin - 80(2023), 12 vom: 02. Feb., Seite 13181-13198 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yıldız, Ayşegül [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Process parameters |
|---|
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
|---|
| doi: |
10.1007/s00289-023-04697-6 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR053430565 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR053430565 | ||
| 003 | DE-627 | ||
| 005 | 20231018064646.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231018s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00289-023-04697-6 |2 doi | |
| 035 | |a (DE-627)SPR053430565 | ||
| 035 | |a (SPR)s00289-023-04697-6-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yıldız, Ayşegül |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of the effect of process parameters on the protein content of silk fibroin |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. | ||
| 520 | |a Abstract Silk fibroin is a natural, protein-based biopolymer produced by silkworm and obtained from silkworm cocoons. It has such features as biocompatibility, biodegradability, high mechanical resistance, low immunogenicity and low inflammatory response. Owing to these properties, it attracts attention in various fields including tissue engineering and drug delivery. The silk fibroin production method basically consists of processes such as degumming, extraction, dialysis, centrifugation, and, if necessary, lyophilization. Since the production of silk fibroin from cocoons includes many steps, there are various parameters that affect the protein content of silk fibroin. In this study, it was aimed to validate the production process steps which mainly affect the silk fibroin content and to determine optimum parameters to obtain silk fibroin with higher protein content. For this purpose, the effects of the type of extraction agent (Ajisawa’s reagent or lithium bromide), the molecular weight cutoff value (MWCO) of the dialysis membrane, the dialysis time, and the centrifugation process were evaluated statistically with one-way analysis of variance method. According to the results, the highest protein content is obtained using, LiBr as extraction agent, 3500 Da as MWCO value of dialysis membrane, 2 days as dialysis time and 532 RCF for 5 min as conditions of centrifugation process. | ||
| 650 | 4 | |a Silk fibroin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Production method |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Process parameters |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Statistical analysis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Mutlu Ağardan, N. Başaran |4 aut | |
| 700 | 1 | |a Acartürk, Füsun |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Polymer bulletin |d Berlin : Springer, 1978 |g 80(2023), 12 vom: 02. Feb., Seite 13181-13198 |w (DE-627)SPR003729788 |w (DE-600)1473175-7 |x 1436-2449 |7 nnns |
| 773 | 1 | 8 | |g volume:80 |g year:2023 |g number:12 |g day:02 |g month:02 |g pages:13181-13198 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00289-023-04697-6 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 80 |j 2023 |e 12 |b 02 |c 02 |h 13181-13198 | ||