Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products
Abstract Fluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the C5 position of the pyrimidine base through a trans-alkene spacer. The substrate efficiency of the labeled nucleotides has been tested in recombinase polymerase amplification (RPA). As a result of RPA, full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) have been formed with a high density of fluorescent label..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
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| Enthalten in: |
Russian journal of bioorganic chemistry - 49(2023), 5 vom: 21. Aug., Seite 1151-1158 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shershov, V. E. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© Pleiades Publishing, Ltd. 2023 |
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| doi: |
10.1134/S1068162023050242 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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SPR052815390 |
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| 520 | |a Abstract Fluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the C5 position of the pyrimidine base through a trans-alkene spacer. The substrate efficiency of the labeled nucleotides has been tested in recombinase polymerase amplification (RPA). As a result of RPA, full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) have been formed with a high density of fluorescent label. | ||
| 700 | 1 | |a Lapa, S. A. |4 aut | |
| 700 | 1 | |a Levashova, A. I. |4 aut | |
| 700 | 1 | |a Shishkin, I. Yu. |4 aut | |
| 700 | 1 | |a Shtylev, G. F. |4 aut | |
| 700 | 1 | |a Shekalova, E. Yu. |4 aut | |
| 700 | 1 | |a Vasiliskov, V. A. |4 aut | |
| 700 | 1 | |a Zasedatelev, A. S. |4 aut | |
| 700 | 1 | |a Kuznetsova, V. E. |4 aut | |
| 700 | 1 | |a Chudinov, A. V. |4 aut | |
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