Species-Level Identification of SARS-CoV-2 by an E Gene Conservative Site
Abstract— The appearance of mutations in the genes encoding the surface proteins of the new type of coronavirus SARS-CoV-2, when it circulates in the host population, makes it difficult to use monoclonal antibodies for its species identification. In such cases, the choice of conservative genetic targets allows identification by molecular biological methods. In this work, previously developed primers specific to the E-gene fragment were tested to detect a new type of coronavirus on six isolates belonging to different genetic variants (the original Wuhan strain, delta and omicron). The choice of a conservative site of the E gene encoding the small transmembrane protein E as a target for reverse transcription with subsequent amplification (RT-PCR) made it possible to detect coronavirus regardless of its subtypes characterized by antigenic heterogeneity in N- and S-proteins. The possibility of species-level identification of the COVID-19 pathogen circulating in Russia is shown, both in the total reaction volume (in a single test tube) and on biological microarrays..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
|---|---|
| Enthalten in: |
Russian journal of bioorganic chemistry - 49(2023), 4 vom: Aug., Seite 912-915 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lapa, S. A. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Anmerkungen: |
© Pleiades Publishing, Ltd. 2023. ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2023, Vol. 49, No. 4, pp. 912–915. © Pleiades Publishing, Ltd., 2023. Russian Text © The Author(s), 2023, published in Bioorganicheskaya Khimiya, 2023, Vol. 49, No. 5, pp. 543–547. |
|---|
| doi: |
10.1134/S1068162023040131 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR052774120 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR052774120 | ||
| 003 | DE-627 | ||
| 005 | 20230818064717.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230818s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1134/S1068162023040131 |2 doi | |
| 035 | |a (DE-627)SPR052774120 | ||
| 035 | |a (SPR)S1068162023040131-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lapa, S. A. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Species-Level Identification of SARS-CoV-2 by an E Gene Conservative Site |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Pleiades Publishing, Ltd. 2023. ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2023, Vol. 49, No. 4, pp. 912–915. © Pleiades Publishing, Ltd., 2023. Russian Text © The Author(s), 2023, published in Bioorganicheskaya Khimiya, 2023, Vol. 49, No. 5, pp. 543–547. | ||
| 520 | |a Abstract— The appearance of mutations in the genes encoding the surface proteins of the new type of coronavirus SARS-CoV-2, when it circulates in the host population, makes it difficult to use monoclonal antibodies for its species identification. In such cases, the choice of conservative genetic targets allows identification by molecular biological methods. In this work, previously developed primers specific to the E-gene fragment were tested to detect a new type of coronavirus on six isolates belonging to different genetic variants (the original Wuhan strain, delta and omicron). The choice of a conservative site of the E gene encoding the small transmembrane protein E as a target for reverse transcription with subsequent amplification (RT-PCR) made it possible to detect coronavirus regardless of its subtypes characterized by antigenic heterogeneity in N- and S-proteins. The possibility of species-level identification of the COVID-19 pathogen circulating in Russia is shown, both in the total reaction volume (in a single test tube) and on biological microarrays. | ||
| 700 | 1 | |a Shingareva, A. A. |4 aut | |
| 700 | 1 | |a Faizuloev, E. B. |4 aut | |
| 700 | 1 | |a Ammour, Yu. I. |4 aut | |
| 700 | 1 | |a Shershov, V. E. |4 aut | |
| 700 | 1 | |a Chudinov, A. V. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Russian journal of bioorganic chemistry |d Moscow : MAIK Nauka/Interperiodica Publ., 2000 |g 49(2023), 4 vom: Aug., Seite 912-915 |w (DE-627)SPR017257387 |w (DE-600)2058156-7 |x 1573-9163 |7 nnns |
| 773 | 1 | 8 | |g volume:49 |g year:2023 |g number:4 |g month:08 |g pages:912-915 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1134/S1068162023040131 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 49 |j 2023 |e 4 |c 08 |h 912-915 | ||