A Regulatory sRNA rli41 is Implicated in Cell Adhesion, Invasion and Pathogenicity in Listeria monocytogenes
Abstract Small regulatory RNAs (sRNAs) play important roles as post-transcriptional regulators throughout the life activities of bacteria. To explore the roles of the regulatory sRNA rli41 identified in Listeria monocytogenes (LM), we analyzed the molecular characteristics and expression profiles of sRNA rli41 during cell infection. Then the deletion strain LM-Δrli41 and complementation strain LM-Δrli41-rli41 were constructed and used to investigate the effects of deficiency of rli41 gene on the adhesion, invasion and pathogenicity of LM. Furthermore, sRNA rli41-regulated potential target genes were predicted in silico, followed by the verification of sRNA-mRNA interaction using a dual plasmid reporter system. The results revealed that sRNA rli41 was highly conserved in LM and its expression level was 15.78-fold up-regulated during cell infection as compared to extracellular environment. Compared with the parental strain and complementation strain, cell adhesion and invasion of the deletion strain were significantly reduced. Moreover, $ LD_{50} $ of LM increased and the pathogenicity weakened significantly due to the deficiency of rli41 genes. Additionally, sRNA rli41 could complementarily base pairing with lmo2178 mRNA 5'-UTR, which could facilitate the expression of peptidoglycan-binding protein Lmo2178, which in turn promotes the cell adhesion, invasion and pathogenicity of LM. Taken together, these findings demonstrated for the first time that the regulatory sRNA rli41 is implicated in cell adhesion, invasion and pathogenicity by modulating the expression of virulence factor Lmo2178 in LM..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:58 |
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| Enthalten in: |
Applied biochemistry and microbiology - 58(2022), Suppl 1 vom: Dez., Seite S47-S56 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, L. X. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© Pleiades Publishing, Inc. 2022. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2022, Vol. 58, Suppl. 1, pp. S47–S56. © Pleiades Publishing, Inc., 2022. |
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| doi: |
10.1134/S0003683822100167 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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SPR051502593 |
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| 520 | |a Abstract Small regulatory RNAs (sRNAs) play important roles as post-transcriptional regulators throughout the life activities of bacteria. To explore the roles of the regulatory sRNA rli41 identified in Listeria monocytogenes (LM), we analyzed the molecular characteristics and expression profiles of sRNA rli41 during cell infection. Then the deletion strain LM-Δrli41 and complementation strain LM-Δrli41-rli41 were constructed and used to investigate the effects of deficiency of rli41 gene on the adhesion, invasion and pathogenicity of LM. Furthermore, sRNA rli41-regulated potential target genes were predicted in silico, followed by the verification of sRNA-mRNA interaction using a dual plasmid reporter system. The results revealed that sRNA rli41 was highly conserved in LM and its expression level was 15.78-fold up-regulated during cell infection as compared to extracellular environment. Compared with the parental strain and complementation strain, cell adhesion and invasion of the deletion strain were significantly reduced. Moreover, $ LD_{50} $ of LM increased and the pathogenicity weakened significantly due to the deficiency of rli41 genes. Additionally, sRNA rli41 could complementarily base pairing with lmo2178 mRNA 5'-UTR, which could facilitate the expression of peptidoglycan-binding protein Lmo2178, which in turn promotes the cell adhesion, invasion and pathogenicity of LM. Taken together, these findings demonstrated for the first time that the regulatory sRNA rli41 is implicated in cell adhesion, invasion and pathogenicity by modulating the expression of virulence factor Lmo2178 in LM. | ||
| 700 | 1 | |a Ji, C. H. |4 aut | |
| 700 | 1 | |a Ning, C. C. |4 aut | |
| 700 | 1 | |a Liu, Y. C. |4 aut | |
| 700 | 1 | |a Li, Z. Y. |4 aut | |
| 700 | 1 | |a Sun, Y. Q. |4 aut | |
| 700 | 1 | |a Xia, X. Z. |4 aut | |
| 700 | 1 | |a Cai, X. P. |4 aut | |
| 700 | 1 | |a Meng, Q. L. |4 aut | |
| 700 | 1 | |a Qiao, J. |4 aut | |
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