Biochip with Agarose Microcells Containing Thermally Separable Primers
Abstract— A method has been developed for the immobilization of short DNA sequences in agarose cells fixed on the surface of a polymer substrate, with their subsequent partial cleavage from agarose when heated while protecting functional properties. The effectiveness of the method was demonstrated on oligonucleotides, which, after cleavage from agarose, retained the ability to participate as primers in the PCR amplification of the sequence site 7 of the exon of the human ABO gene..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Russian journal of bioorganic chemistry - 48(2022), 4 vom: Aug., Seite 858-863 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zolotov, A. M. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© Pleiades Publishing, Ltd. 2022. ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2022, Vol. 48, No. 4, pp. 858–863. © Pleiades Publishing, Ltd., 2022. Russian Text © The Author(s), 2022, published in Bioorganicheskaya Khimiya, 2022, Vol. 48, No. 4, pp. 599–605. |
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| doi: |
10.1134/S1068162022040203 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract— A method has been developed for the immobilization of short DNA sequences in agarose cells fixed on the surface of a polymer substrate, with their subsequent partial cleavage from agarose when heated while protecting functional properties. The effectiveness of the method was demonstrated on oligonucleotides, which, after cleavage from agarose, retained the ability to participate as primers in the PCR amplification of the sequence site 7 of the exon of the human ABO gene. | ||
| 700 | 1 | |a Miftakhov, R. A. |4 aut | |
| 700 | 1 | |a Ikonnikova, A. Y. |4 aut | |
| 700 | 1 | |a Lapa, S. A. |4 aut | |
| 700 | 1 | |a Kuznetsova, V. E. |4 aut | |
| 700 | 1 | |a Vasiliskov, V. A. |4 aut | |
| 700 | 1 | |a Shershov, V. E. |4 aut | |
| 700 | 1 | |a Zasedatelev, A. S. |4 aut | |
| 700 | 1 | |a Nasedkina, T. V. |4 aut | |
| 700 | 1 | |a Chudinov, A. V. |4 aut | |
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