CASP5 and CR1 as potential biomarkers for Kawasaki disease: an Integrated Bioinformatics-Experimental Study
Background Kawasaki disease (KD) is a pediatric inflammatory disorder causes coronary artery complications. The disease overlapping manifestations with a set of symptomatically like diseases such as bacterial and viral infections, juvenile idiopathic arthritis, Henoch-Schönlein purpura, infection of unknown etiology, group-A streptococcal and adenoviral infections, and incomplete KD could lead to misdiagnosis of the disease. Methods In the present study, we applied weighted gene co-expression network analysis (WGCNA) to identify network modules of co-expressed genes in GSE73464 and also, limma package was used to identify the differentially expressed genes (DEGs) in KD expression arrays composed of GSE73464, GSE18606, GSE109351, and GSE68004. By merging the results of WGCNA and limma, we detected hub genes. Then, analyzed the peripheral blood mononuclear cells (PBMCs) of 16 patients and 8 control subjects using Real-Time Polymerase Chain Reaction (RT-PCR) to evaluate the previous results. Results We assessed the diagnostic potency of the screened genes by plotting the area under curve (AUC). We finally identified 2 genes CASP5(Caspase 5) and CR1(Complement C3b/C4b Receptor 1) which were shown to potentially discriminate KD from other similar diseases and also from healthy people. Conclusions The results of RT-PCR and AUC confirmed the diagnostic potentials of two suggested biomarkers for KD..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
BMC pediatrics - 21(2021), 1 vom: 11. Dez. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rahmati, Yazdan [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| Anmerkungen: |
© The Author(s) 2021 |
|---|
| doi: |
10.1186/s12887-021-03003-5 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR050348310 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR050348310 | ||
| 003 | DE-627 | ||
| 005 | 20230509095632.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230507s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s12887-021-03003-5 |2 doi | |
| 035 | |a (DE-627)SPR050348310 | ||
| 035 | |a (SPR)s12887-021-03003-5-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Rahmati, Yazdan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a CASP5 and CR1 as potential biomarkers for Kawasaki disease: an Integrated Bioinformatics-Experimental Study |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s) 2021 | ||
| 520 | |a Background Kawasaki disease (KD) is a pediatric inflammatory disorder causes coronary artery complications. The disease overlapping manifestations with a set of symptomatically like diseases such as bacterial and viral infections, juvenile idiopathic arthritis, Henoch-Schönlein purpura, infection of unknown etiology, group-A streptococcal and adenoviral infections, and incomplete KD could lead to misdiagnosis of the disease. Methods In the present study, we applied weighted gene co-expression network analysis (WGCNA) to identify network modules of co-expressed genes in GSE73464 and also, limma package was used to identify the differentially expressed genes (DEGs) in KD expression arrays composed of GSE73464, GSE18606, GSE109351, and GSE68004. By merging the results of WGCNA and limma, we detected hub genes. Then, analyzed the peripheral blood mononuclear cells (PBMCs) of 16 patients and 8 control subjects using Real-Time Polymerase Chain Reaction (RT-PCR) to evaluate the previous results. Results We assessed the diagnostic potency of the screened genes by plotting the area under curve (AUC). We finally identified 2 genes CASP5(Caspase 5) and CR1(Complement C3b/C4b Receptor 1) which were shown to potentially discriminate KD from other similar diseases and also from healthy people. Conclusions The results of RT-PCR and AUC confirmed the diagnostic potentials of two suggested biomarkers for KD. | ||
| 650 | 4 | |a Gene expression |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hub genes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a RT-PCR |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a ROC curve |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a WGCNA |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Mollanoori, Hasan |4 aut | |
| 700 | 1 | |a Najafi, Sajad |4 aut | |
| 700 | 1 | |a Esmaeili, Sajjad |4 aut | |
| 700 | 1 | |a Alivand, Mohammad Reza |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BMC pediatrics |d London : BioMed Central, 2001 |g 21(2021), 1 vom: 11. Dez. |w (DE-627)SPR027744639 |w (DE-600)2041342-7 |x 1471-2431 |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2021 |g number:1 |g day:11 |g month:12 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1186/s12887-021-03003-5 |z kostenfrei |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 21 |j 2021 |e 1 |b 11 |c 12 | ||