Lactiplantibacillus plantarum PGB02 Improved Serum Cholesterol Profile by Tweaking Genes Involved in Cholesterol Homeostasis in Male Swiss Albino Mice
Abstract The effect of Lactiplantibacillus plantarum PGB02 isolated from buttermilk on serum cholesterol profile of normal and hypercholesterolemic mice was evaluated. Further changes in the expression of mice genes were determined. The hypercholesterolemia was induced in experimental mice by feeding high cholesterol and fat diet. Serum cholesterol parameters, physical parameters, cholic acid excretion, and cholesterol metabolism related gene expression analysis was carried out. L. plantarum PGB02 efficiently reduced total cholesterol, triglycerides, and LDL-cholesterol and improved HDL-cholesterol in hypercholesterolaemic mice. Body weight was reduced and fecal cholic acid increased in probiotic treatment groups. Gene expression analysis revealed that L. plantarum PGB02 up-regulated the expression of LDL receptors, CYP7A1, ABCA1, ABCG5, ABCG8, and down-regulated the expression of FXR and NPC1L1 genes. Summarizing the mechanism, L. plantarum PGB02 improved hypercholesterolemia by increasing bile acid synthesis and excretion, reducing exogeneous cholesterol absorption from the intestine, and increased LDL clearance through upregulation of LDL-receptors. The present study has given insight into the mechanism of serum cholesterol reduction by bile salt hydrolase positive L. plantarum PGB02 in mice. L. plantarum PGB02 reduced the serum cholesterol level through increased bile acid synthesis and deconjugation and reduced absorption of cholesterol in the intestine. Isolate PGB02 shown cholesterol removal potential as good as statin..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:195 |
|---|---|
| Enthalten in: |
Applied biochemistry and biotechnology - 195(2022), 5 vom: 27. Dez., Seite 3180-3193 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dhameliya, Hiren A. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Bile acid metabolism |
|---|
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
|---|
| doi: |
10.1007/s12010-022-04307-7 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR050268708 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR050268708 | ||
| 003 | DE-627 | ||
| 005 | 20230503064712.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230503s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12010-022-04307-7 |2 doi | |
| 035 | |a (DE-627)SPR050268708 | ||
| 035 | |a (SPR)s12010-022-04307-7-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Dhameliya, Hiren A. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lactiplantibacillus plantarum PGB02 Improved Serum Cholesterol Profile by Tweaking Genes Involved in Cholesterol Homeostasis in Male Swiss Albino Mice |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. | ||
| 520 | |a Abstract The effect of Lactiplantibacillus plantarum PGB02 isolated from buttermilk on serum cholesterol profile of normal and hypercholesterolemic mice was evaluated. Further changes in the expression of mice genes were determined. The hypercholesterolemia was induced in experimental mice by feeding high cholesterol and fat diet. Serum cholesterol parameters, physical parameters, cholic acid excretion, and cholesterol metabolism related gene expression analysis was carried out. L. plantarum PGB02 efficiently reduced total cholesterol, triglycerides, and LDL-cholesterol and improved HDL-cholesterol in hypercholesterolaemic mice. Body weight was reduced and fecal cholic acid increased in probiotic treatment groups. Gene expression analysis revealed that L. plantarum PGB02 up-regulated the expression of LDL receptors, CYP7A1, ABCA1, ABCG5, ABCG8, and down-regulated the expression of FXR and NPC1L1 genes. Summarizing the mechanism, L. plantarum PGB02 improved hypercholesterolemia by increasing bile acid synthesis and excretion, reducing exogeneous cholesterol absorption from the intestine, and increased LDL clearance through upregulation of LDL-receptors. The present study has given insight into the mechanism of serum cholesterol reduction by bile salt hydrolase positive L. plantarum PGB02 in mice. L. plantarum PGB02 reduced the serum cholesterol level through increased bile acid synthesis and deconjugation and reduced absorption of cholesterol in the intestine. Isolate PGB02 shown cholesterol removal potential as good as statin. | ||
| 650 | 4 | |a Cardiovascular diseases |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bile acid metabolism |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hypercholesterolemia |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hyperlipidemia |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Probiotics |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Solanki, Jyoti D. |4 aut | |
| 700 | 1 | |a Patel, Dhaval T. |4 aut | |
| 700 | 1 | |a George, Linz-buoy |4 aut | |
| 700 | 1 | |a Thakkar, Vasudev R. |4 aut | |
| 700 | 1 | |a Subramanian, R. B. |0 (orcid)0000-0002-4322-9513 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied biochemistry and biotechnology |d Berlin : Springer, 1976 |g 195(2022), 5 vom: 27. Dez., Seite 3180-3193 |w (DE-627)SPR02354368X |w (DE-600)2072711-2 |x 1559-0291 |7 nnns |
| 773 | 1 | 8 | |g volume:195 |g year:2022 |g number:5 |g day:27 |g month:12 |g pages:3180-3193 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s12010-022-04307-7 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 195 |j 2022 |e 5 |b 27 |c 12 |h 3180-3193 | ||