Nucleotide diversity and molecular characterization of soluble starch synthase I gene in wheat and its ancestral species
Abstract Starch, the primary determinant of wheat grain yield, contributes 65–75% grain dry weight and 80% of the endosperm dry weight. The starch biosynthetic pathway is regulated by several enzymes, of which the soluble starch synthase (SS) is the most crucial enzyme and is sensitive to environmental change. In the present study, we targeted identifying the unique haplotypes of SSI gene in 17 different wild and cultivated genotypes of wheat, selected for abiotic stress tolerance. Four unique SNPs corresponding to non-synonymous substitution were identified from wild progenitor species, Aegilops tauschii (accessions pau14102 and pau3747), Ae. speltoides (pau15081) and T. dicoccoides (pau7107), each present in exon-1. However, two unique SNPs corresponding to non-synonymous substitution were identified from cultivated wheats, Impala and C591, present in exon-2 and exon-4, respectively. These SNPs correspond to the N-terminal half of the GT5 domain in exon 1–6 and are expected as novel haplotypes contributing to better grain filling and starch deposition under changing environmental conditions. The positive effects of these haplotypes need further validation through molecular and phenotypic evaluation..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Journal of plant biochemistry and biotechnology - 32(2022), 1 vom: 21. Mai, Seite 92-105 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Singh, Mehakdeep [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Society for Plant Biochemistry and Biotechnology 2022 |
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| doi: |
10.1007/s13562-022-00785-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Nucleotide diversity and molecular characterization of soluble starch synthase I gene in wheat and its ancestral species |
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| 520 | |a Abstract Starch, the primary determinant of wheat grain yield, contributes 65–75% grain dry weight and 80% of the endosperm dry weight. The starch biosynthetic pathway is regulated by several enzymes, of which the soluble starch synthase (SS) is the most crucial enzyme and is sensitive to environmental change. In the present study, we targeted identifying the unique haplotypes of SSI gene in 17 different wild and cultivated genotypes of wheat, selected for abiotic stress tolerance. Four unique SNPs corresponding to non-synonymous substitution were identified from wild progenitor species, Aegilops tauschii (accessions pau14102 and pau3747), Ae. speltoides (pau15081) and T. dicoccoides (pau7107), each present in exon-1. However, two unique SNPs corresponding to non-synonymous substitution were identified from cultivated wheats, Impala and C591, present in exon-2 and exon-4, respectively. These SNPs correspond to the N-terminal half of the GT5 domain in exon 1–6 and are expected as novel haplotypes contributing to better grain filling and starch deposition under changing environmental conditions. The positive effects of these haplotypes need further validation through molecular and phenotypic evaluation. | ||
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| 700 | 1 | |a Kaur, Amandeep |0 (orcid)0000-0002-9140-3424 |4 aut | |
| 700 | 1 | |a Yadav, Inderjit Singh |4 aut | |
| 700 | 1 | |a Sharma, Priti |4 aut | |
| 700 | 1 | |a Chhuneja, Parveen |0 (orcid)0000-0002-8599-9479 |4 aut | |
| 700 | 1 | |a Singh, Kuldeep |4 aut | |
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