Associations between inflammation-related LL-37 with subgingival microbial dysbiosis in rheumatoid arthritis patients
Objective This study investigated the subgingival microbial profile of rheumatoid arthritis (RA) patients and its associations with disease parameters and the inflammation-related antimicrobial peptide, LL-37. Methods RA and non-RA (NRA) patients were assessed for periodontal status and divided into periodontitis (CP), gingivitis (G), and healthy (H) groups. Subgingival plaque 16s rRNA gene sequencing data was processed and analyzed using the CLC Genomic Workbench (Qiagen). Bacterial diversity and co-occurrence patterns were examined. Differential abundance between groups was also investigated. Associations between bacterial genera with disease parameters and LL-37 levels were explored qualitatively using canonical correlation analysis. Results Subgingival microbial community clustered in CP status. Co-occurrence network in NRA-H was dominated by health-associated genera, while the rest of the networks’ key genera were both health- and disease-associated. RA-CP displayed highly inter-generic networks with a statistically significant increase in periodontal disease-associated genera (p<0.05). In NRA-H, disease parameters and LL-37 were correlated positively with disease-associated genera while negatively with health-associated genera. However, in the remaining groups, mixed positive and negative correlations were noted with genera. Conclusion RA patients demonstrated subgingival microbial dysbiosis where the bacteria networks were dominated by health- and disease-associated genera. Mixed correlations with disease parameters and LL-37 levels were noted. Clinical relevance The subgingival microbial dysbiosis in RA may predispose these patients to developing periodontal inflammation with an associated detrimental effect on host immune responses. Routine periodontal assessment may allow initiation of treatment strategies to minimize the effects of gingival inflammation on the existing heightened immune response present in RA patients..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
|---|---|
| Enthalten in: |
Clinical Oral Investigations - 26(2022), 5 vom: 07. März, Seite 4161-4172 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Cheah, Chia Wei [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Dysbiosis |
|---|
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
|---|
| doi: |
10.1007/s00784-022-04388-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR046911618 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR046911618 | ||
| 003 | DE-627 | ||
| 005 | 20230519163120.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220506s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00784-022-04388-y |2 doi | |
| 035 | |a (DE-627)SPR046911618 | ||
| 035 | |a (SPR)s00784-022-04388-y-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Cheah, Chia Wei |e verfasserin |0 (orcid)0000-0002-5795-4249 |4 aut | |
| 245 | 1 | 0 | |a Associations between inflammation-related LL-37 with subgingival microbial dysbiosis in rheumatoid arthritis patients |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 | ||
| 520 | |a Objective This study investigated the subgingival microbial profile of rheumatoid arthritis (RA) patients and its associations with disease parameters and the inflammation-related antimicrobial peptide, LL-37. Methods RA and non-RA (NRA) patients were assessed for periodontal status and divided into periodontitis (CP), gingivitis (G), and healthy (H) groups. Subgingival plaque 16s rRNA gene sequencing data was processed and analyzed using the CLC Genomic Workbench (Qiagen). Bacterial diversity and co-occurrence patterns were examined. Differential abundance between groups was also investigated. Associations between bacterial genera with disease parameters and LL-37 levels were explored qualitatively using canonical correlation analysis. Results Subgingival microbial community clustered in CP status. Co-occurrence network in NRA-H was dominated by health-associated genera, while the rest of the networks’ key genera were both health- and disease-associated. RA-CP displayed highly inter-generic networks with a statistically significant increase in periodontal disease-associated genera (p<0.05). In NRA-H, disease parameters and LL-37 were correlated positively with disease-associated genera while negatively with health-associated genera. However, in the remaining groups, mixed positive and negative correlations were noted with genera. Conclusion RA patients demonstrated subgingival microbial dysbiosis where the bacteria networks were dominated by health- and disease-associated genera. Mixed correlations with disease parameters and LL-37 levels were noted. Clinical relevance The subgingival microbial dysbiosis in RA may predispose these patients to developing periodontal inflammation with an associated detrimental effect on host immune responses. Routine periodontal assessment may allow initiation of treatment strategies to minimize the effects of gingival inflammation on the existing heightened immune response present in RA patients. | ||
| 650 | 4 | |a Inflammation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a LL-37 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Subgingival microbial |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Dysbiosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rheumatoid arthritis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Al-Maleki, Anis Rageh |4 aut | |
| 700 | 1 | |a Vaithilingam, Rathna Devi |4 aut | |
| 700 | 1 | |a Vadivelu, Jamuna |4 aut | |
| 700 | 1 | |a Sockalingam, Sargunan |4 aut | |
| 700 | 1 | |a Baharuddin, Nor Adinar |4 aut | |
| 700 | 1 | |a Bartold, Peter Mark |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Clinical Oral Investigations |d Springer-Verlag, 2001 |g 26(2022), 5 vom: 07. März, Seite 4161-4172 |w (DE-627)SPR007794231 |7 nnns |
| 773 | 1 | 8 | |g volume:26 |g year:2022 |g number:5 |g day:07 |g month:03 |g pages:4161-4172 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00784-022-04388-y |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |d 26 |j 2022 |e 5 |b 07 |c 03 |h 4161-4172 | ||