Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-$ Fe_{2} %$ O_{3} $ nanoparticles
A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ $ Fe_{2} %$ O_{3} $ nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ $ Fe_{2} %$ O_{3} $ nanoparticles, the color change of $ H_{2} %$ O_{2} $ included 3,3′,5,5′-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained. Graphical abstract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:188 |
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| Enthalten in: |
Microchimica acta - 188(2021), 10 vom: 09. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Büyüksünetçi, Yudum Tepeli [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
COVID-19 |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 |
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| doi: |
10.1007/s00604-021-04989-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ $ Fe_{2} %$ O_{3} $ nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ $ Fe_{2} %$ O_{3} $ nanoparticles, the color change of $ H_{2} %$ O_{2} $ included 3,3′,5,5′-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained. Graphical abstract | ||
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