Aging and airway inflammation
Background Since little is known of airways inflammation in the elderly, we have carried out a study to explore the presence of some inflammatory markers in the airways of healthy subjects of different ages using a non-invasive method which is particularly suitable for aged people. Objective The aim of this work was to investigate whether parameters, including (1) pH, IL-8 and TNF-α in exhaled breath condensate (EBC), (2) exhaled nitric oxide levels (NO), and (3) inflammatory cell profile in induced sputum, are age-related. Materials and methods Thirty healthy adults (10 subjects below the age of 30 [A], 10 subjects between 30 and 60 years [B], and 10 subjects over 60 years of age [C]), were enrolled in the study. IL-8 and TNF-α levels were measured in breath condensate. Exhaled pH was measured after deaeration/decarbonation by means of a pH-meter. A rapid-response chemiluminescence NO analyzer was used to quantify NO. Induced sputum was collected, homogenized with dithiothreitol, and cytospins for differential cell were produced. Results The levels of IL-8 and TNF-α in EBC, the levels of exhaled NO, and the percentage of neutrophils in induced sputum were significantly elevated in C and B compared with A; the EBC pH level was significantly reduced in C and B compared with A. The EBC levels of IL-8, TNF-α, pH, the level of exhaled NO, and the percentage of neutrophils correlated significantly with age. Conclusion This study has shown the presence of age-related airways inflammation in healthy subjects..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Aging clinical and experimental research - 25(2013), 3 vom: 18. Mai, Seite 239-245 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Carpagnano, Giovanna Elisiana [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Airways inflammation |
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| Anmerkungen: |
© Springer International Publishing Switzerland 2013 |
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| doi: |
10.1007/s40520-013-0040-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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SPR036604771 |
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| 520 | |a Background Since little is known of airways inflammation in the elderly, we have carried out a study to explore the presence of some inflammatory markers in the airways of healthy subjects of different ages using a non-invasive method which is particularly suitable for aged people. Objective The aim of this work was to investigate whether parameters, including (1) pH, IL-8 and TNF-α in exhaled breath condensate (EBC), (2) exhaled nitric oxide levels (NO), and (3) inflammatory cell profile in induced sputum, are age-related. Materials and methods Thirty healthy adults (10 subjects below the age of 30 [A], 10 subjects between 30 and 60 years [B], and 10 subjects over 60 years of age [C]), were enrolled in the study. IL-8 and TNF-α levels were measured in breath condensate. Exhaled pH was measured after deaeration/decarbonation by means of a pH-meter. A rapid-response chemiluminescence NO analyzer was used to quantify NO. Induced sputum was collected, homogenized with dithiothreitol, and cytospins for differential cell were produced. Results The levels of IL-8 and TNF-α in EBC, the levels of exhaled NO, and the percentage of neutrophils in induced sputum were significantly elevated in C and B compared with A; the EBC pH level was significantly reduced in C and B compared with A. The EBC levels of IL-8, TNF-α, pH, the level of exhaled NO, and the percentage of neutrophils correlated significantly with age. Conclusion This study has shown the presence of age-related airways inflammation in healthy subjects. | ||
| 650 | 4 | |a Elderly |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Exhaled breath condensate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Airways inflammation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Induced sputum |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Exhaled nitric oxide |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Turchiarelli, Viviana |4 aut | |
| 700 | 1 | |a Spanevello, Antonio |4 aut | |
| 700 | 1 | |a Palladino, Grazia Pia |4 aut | |
| 700 | 1 | |a Barbaro, Maria Pia Foschino |4 aut | |
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