Genotoxicity and effect on early stage proliferation of osteoprogenitor cells on amino-group functionalized titanium implant surface: an in vitro test
Abstract Genotoxicity and effect on early stage proliferation of osteoprogenitor cells of titanium surfaces modified by heptylamine functionalization through Plasma Enhanced Chemical Vapor Deposition (PECVD) were evaluated in current study. Mouse embryo osteoprogenitor MC3T3-E1 cells were grown on titanium surfaces of all the groups. The CCK-8 assay showed the growths of MC3T3-E1 cells on treated titanium disks were not inhibited compared with those on pure titanium groups. Result of Single Cell Gel Electrophoresis (SCGE) test indicated the treated groups showed no significant differences in tail length, tail DNA%, and tail moment compared with pure titanium group. Rate of micronucleus, nucleoplamic buds and nuclear blebs showed no significant differences between treated groups and pure titanium group. Our research exhibited that: The amino-group functionalized titanium surface has neither genotoxicity nor detrimental effects on early-stage proliferation towards osteoprogenitor cells in vitro, thus offer a theoretic support for the clinical use of such functionalized titanium as implant surface..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Molecular & cellular toxicology - 12(2016), 2 vom: Juni, Seite 167-175 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Guo, Tian-qi [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Genotoxicity |
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| Anmerkungen: |
© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht 2016 |
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| doi: |
10.1007/s13273-016-0021-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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SPR031118267 |
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| 245 | 1 | 0 | |a Genotoxicity and effect on early stage proliferation of osteoprogenitor cells on amino-group functionalized titanium implant surface: an in vitro test |
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| 520 | |a Abstract Genotoxicity and effect on early stage proliferation of osteoprogenitor cells of titanium surfaces modified by heptylamine functionalization through Plasma Enhanced Chemical Vapor Deposition (PECVD) were evaluated in current study. Mouse embryo osteoprogenitor MC3T3-E1 cells were grown on titanium surfaces of all the groups. The CCK-8 assay showed the growths of MC3T3-E1 cells on treated titanium disks were not inhibited compared with those on pure titanium groups. Result of Single Cell Gel Electrophoresis (SCGE) test indicated the treated groups showed no significant differences in tail length, tail DNA%, and tail moment compared with pure titanium group. Rate of micronucleus, nucleoplamic buds and nuclear blebs showed no significant differences between treated groups and pure titanium group. Our research exhibited that: The amino-group functionalized titanium surface has neither genotoxicity nor detrimental effects on early-stage proliferation towards osteoprogenitor cells in vitro, thus offer a theoretic support for the clinical use of such functionalized titanium as implant surface. | ||
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| 700 | 1 | |a Li, Xue |4 aut | |
| 700 | 1 | |a Zhou, Yan-min |4 aut | |
| 700 | 1 | |a Zhao, Jing-hui |4 aut | |
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