The signature of liver cancer in immune cells DNA methylation
Background The idea that changes to the host immune system are critical for cancer progression was proposed a century ago and recently regained experimental support. Results Herein, the hypothesis that hepatocellular carcinoma (HCC) leaves a molecular signature in the host peripheral immune system was tested by profiling DNA methylation in peripheral blood mononuclear cells (PBMC) and T cells from a discovery cohort (n = 69) of healthy controls, chronic hepatitis, and HCC using Illumina 450K platform and was validated in two validation sets (n = 80 and n = 48) using pyrosequencing. Conclusions The study reveals a broad signature of hepatocellular carcinoma in PBMC and T cells DNA methylation which discriminates early HCC stage from chronic hepatitis B and C and healthy controls, intensifies with progression of HCC, and is highly enriched in immune function-related genes such as PD-1, a current cancer immunotherapy target. These data also support the feasibility of using these profiles for early detection of HCC..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Clinical epigenetics - 10(2018), 1 vom: 18. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Yonghong [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
DNA methylation |
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| Anmerkungen: |
© The Author(s). 2018 |
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| doi: |
10.1186/s13148-017-0436-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Background The idea that changes to the host immune system are critical for cancer progression was proposed a century ago and recently regained experimental support. Results Herein, the hypothesis that hepatocellular carcinoma (HCC) leaves a molecular signature in the host peripheral immune system was tested by profiling DNA methylation in peripheral blood mononuclear cells (PBMC) and T cells from a discovery cohort (n = 69) of healthy controls, chronic hepatitis, and HCC using Illumina 450K platform and was validated in two validation sets (n = 80 and n = 48) using pyrosequencing. Conclusions The study reveals a broad signature of hepatocellular carcinoma in PBMC and T cells DNA methylation which discriminates early HCC stage from chronic hepatitis B and C and healthy controls, intensifies with progression of HCC, and is highly enriched in immune function-related genes such as PD-1, a current cancer immunotherapy target. These data also support the feasibility of using these profiles for early detection of HCC. | ||
| 650 | 4 | |a DNA methylation |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Zhou, Rudy |4 aut | |
| 700 | 1 | |a Dymov, Sergiy |4 aut | |
| 700 | 1 | |a Li, Kang |4 aut | |
| 700 | 1 | |a Li, Ning |4 aut | |
| 700 | 1 | |a Szyf, Moshe |0 (orcid)0000-0003-3881-8214 |4 aut | |
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