PIRCh-seq: functional classification of non-coding RNAs associated with distinct histone modifications
Abstract We develop PIRCh-seq, a method which enables a comprehensive survey of chromatin-associated RNAs in a histone modification-specific manner. We identify hundreds of chromatin-associated RNAs in several cell types with substantially less contamination by nascent transcripts. Non-coding RNAs are found enriched on chromatin and are classified into functional groups based on the patterns of their association with specific histone modifications. We find single-stranded RNA bases are more chromatin-associated, and we discover hundreds of allele-specific RNA-chromatin interactions. These results provide a unique resource to globally study the functions of chromatin-associated lncRNAs and elucidate the basic mechanisms of chromatin-RNA interactions..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Genome biology - 20(2019), 1 vom: 20. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fang, Jingwen [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
© The Author(s). 2019 |
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| doi: |
10.1186/s13059-019-1880-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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SPR030036380 |
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| 245 | 1 | 0 | |a PIRCh-seq: functional classification of non-coding RNAs associated with distinct histone modifications |
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| 520 | |a Abstract We develop PIRCh-seq, a method which enables a comprehensive survey of chromatin-associated RNAs in a histone modification-specific manner. We identify hundreds of chromatin-associated RNAs in several cell types with substantially less contamination by nascent transcripts. Non-coding RNAs are found enriched on chromatin and are classified into functional groups based on the patterns of their association with specific histone modifications. We find single-stranded RNA bases are more chromatin-associated, and we discover hundreds of allele-specific RNA-chromatin interactions. These results provide a unique resource to globally study the functions of chromatin-associated lncRNAs and elucidate the basic mechanisms of chromatin-RNA interactions. | ||
| 700 | 1 | |a Ma, Qing |4 aut | |
| 700 | 1 | |a Chu, Ci |4 aut | |
| 700 | 1 | |a Huang, Beibei |4 aut | |
| 700 | 1 | |a Li, Lingjie |4 aut | |
| 700 | 1 | |a Cai, Pengfei |4 aut | |
| 700 | 1 | |a Batista, Pedro J. |4 aut | |
| 700 | 1 | |a Tolentino, Karen Erisse Martin |4 aut | |
| 700 | 1 | |a Xu, Jin |4 aut | |
| 700 | 1 | |a Li, Rui |4 aut | |
| 700 | 1 | |a Du, Pengcheng |4 aut | |
| 700 | 1 | |a Qu, Kun |4 aut | |
| 700 | 1 | |a Chang, Howard Y. |4 aut | |
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