Development of a Sensitive Monoclonal Antibody–Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Monensin in Edible Chicken Tissues
Abstract To monitor monensin (MON) residues in chicken tissues, a monoclonal antibody (mAb)–based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed in this study. The haptenMON was obtained by adding hydrochloric acid to a MON salt, and then was coupled with carrier protein. After the inoculation of female Balb/c mice and cell fusions, one cell line, MON/4C9, was obtained. The MON/4C9 antibody exhibited the ability to specifically recognize MON with $ IC_{50} $ 1.28 μg $ L^{−1} $.Based on this mAb, an optimized ic-ELISA protocol was performed using only methanol-water (8:2, v/v) in chicken tissue samples. The limits of detection and the limits of quantification of MON in various sample matrices varied from 0.38 to 1.01 μg $ kg^{−1} $. The recoveries ranged from 71.9 to 116.9% in chicken tissues, and the intra- and inter-assay CVs were all less than 18%. Moreover, the developed method also exhibited a positive correlation with the results of HPLC-MS conducted on the samples. These results suggest that the prepared mAb and the developed ic-ELISA method will be a useful tool for detecting MON in chicken tissues..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
|---|---|
| Enthalten in: |
Food analytical methods - 12(2019), 6 vom: 05. März, Seite 1479-1486 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Langhong [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Edible chicken tissues |
|---|
| Anmerkungen: |
© Springer Science+Business Media, LLC, part of Springer Nature 2019 |
|---|
| doi: |
10.1007/s12161-019-01461-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR025261150 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR025261150 | ||
| 003 | DE-627 | ||
| 005 | 20230519172513.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201007s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12161-019-01461-3 |2 doi | |
| 035 | |a (DE-627)SPR025261150 | ||
| 035 | |a (SPR)s12161-019-01461-3-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Langhong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of a Sensitive Monoclonal Antibody–Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Monensin in Edible Chicken Tissues |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Springer Science+Business Media, LLC, part of Springer Nature 2019 | ||
| 520 | |a Abstract To monitor monensin (MON) residues in chicken tissues, a monoclonal antibody (mAb)–based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed in this study. The haptenMON was obtained by adding hydrochloric acid to a MON salt, and then was coupled with carrier protein. After the inoculation of female Balb/c mice and cell fusions, one cell line, MON/4C9, was obtained. The MON/4C9 antibody exhibited the ability to specifically recognize MON with $ IC_{50} $ 1.28 μg $ L^{−1} $.Based on this mAb, an optimized ic-ELISA protocol was performed using only methanol-water (8:2, v/v) in chicken tissue samples. The limits of detection and the limits of quantification of MON in various sample matrices varied from 0.38 to 1.01 μg $ kg^{−1} $. The recoveries ranged from 71.9 to 116.9% in chicken tissues, and the intra- and inter-assay CVs were all less than 18%. Moreover, the developed method also exhibited a positive correlation with the results of HPLC-MS conducted on the samples. These results suggest that the prepared mAb and the developed ic-ELISA method will be a useful tool for detecting MON in chicken tissues. | ||
| 650 | 4 | |a Monensin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Monoclonal antibody |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Indirect competitive enzyme-linked immunosorbent assay |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Edible chicken tissues |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Pan, Yuanhu |4 aut | |
| 700 | 1 | |a Tao, Yanfei |4 aut | |
| 700 | 1 | |a Chen, Donmei |4 aut | |
| 700 | 1 | |a Wang, Yulian |4 aut | |
| 700 | 1 | |a Wang, Xu |4 aut | |
| 700 | 1 | |a Liu, Zhenli |4 aut | |
| 700 | 1 | |a Peng, Dapeng |4 aut | |
| 700 | 1 | |a Yuan, Zonghui |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food analytical methods |d New York, NY : Springer, 2008 |g 12(2019), 6 vom: 05. März, Seite 1479-1486 |w (DE-627)SPR025083481 |w (DE-600)2424728-5 |x 1936-976X |7 nnns |
| 773 | 1 | 8 | |g volume:12 |g year:2019 |g number:6 |g day:05 |g month:03 |g pages:1479-1486 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s12161-019-01461-3 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |d 12 |j 2019 |e 6 |b 05 |c 03 |h 1479-1486 | ||