Rhizobium pakistanensis sp. nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan
Abstract A Gram-negative, white, non-motile, rod shaped bacterial strain BN-$ 19^{T} $ was isolated from a root nodule of groundnut (Arachis hypogaea) in Pakistan. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain BN-$ 19^{T} $ formed a subclade in the genus Rhizobium together with Rhizobium alkalisoli CCBAU $ 01393^{T} $, Rhizobium vignae CCBAU $ 05176^{T} $, Rhizobium huautlense $ SO2^{T} $ and Rhizobium tarimense PL-$ 41^{T} $ with sequence similarities of 97.5, 97.3, 97.2 and 97.1 % respectively. Sequence analysis of housekeeping genes atpD, glnII and recA (with sequence similarities of ≤92 %) confirmed the unique position of BN-$ 19^{T} $ in the genus Rhizobium. DNA–DNA relatedness between the strain BN-$ 19^{T} $ and R. alkalisoli CCBAU $ 01393^{T} $, R. vignae CCBAU $ 05176^{T} $, R. huautlense $ SO2^{T} $ and R. tarimense PL-$ 41^{T} $ were 20.6, 22.5, 15.9 and 20.5 % respectively, further confirming that BN-$ 19^{T} $ represents a novel species in the genus Rhizobium. The DNA G + C content was 60.1 mol%. The dominant fatty acids of strain BN-$ 19^{T} $ were $ C_{19:0} $ cyclo ω8c, summed feature 2 ($ C_{14:0} $ 3OH and/or $ C_{16:1} $ iso I) and summed feature 8 ($ C_{18:1} $ω7c). Some phenotypic features also differentiate the strain BN-$ 19^{T} $ from the related species. On the basis of these results, strain BN-$ 19^{T} $ is considered to represent a novel species in the genus Rhizobium, for which the name Rhizobium pakistanensis sp. nov. is proposed. The type strain is BN-$ 19^{T} $ (=LMG $ 27895^{T} $ = CCBAU $ 101086^{T} $)..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:107 |
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| Enthalten in: |
Antonie van Leeuwenhoek - 107(2014), 1 vom: 13. Nov., Seite 281-290 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khalid, Rabia [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
| doi: |
10.1007/s10482-014-0326-x |
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| Förderinstitution / Projekttitel: |
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SPR010376666 |
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| 245 | 1 | 0 | |a Rhizobium pakistanensis sp. nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan |
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| 520 | |a Abstract A Gram-negative, white, non-motile, rod shaped bacterial strain BN-$ 19^{T} $ was isolated from a root nodule of groundnut (Arachis hypogaea) in Pakistan. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain BN-$ 19^{T} $ formed a subclade in the genus Rhizobium together with Rhizobium alkalisoli CCBAU $ 01393^{T} $, Rhizobium vignae CCBAU $ 05176^{T} $, Rhizobium huautlense $ SO2^{T} $ and Rhizobium tarimense PL-$ 41^{T} $ with sequence similarities of 97.5, 97.3, 97.2 and 97.1 % respectively. Sequence analysis of housekeeping genes atpD, glnII and recA (with sequence similarities of ≤92 %) confirmed the unique position of BN-$ 19^{T} $ in the genus Rhizobium. DNA–DNA relatedness between the strain BN-$ 19^{T} $ and R. alkalisoli CCBAU $ 01393^{T} $, R. vignae CCBAU $ 05176^{T} $, R. huautlense $ SO2^{T} $ and R. tarimense PL-$ 41^{T} $ were 20.6, 22.5, 15.9 and 20.5 % respectively, further confirming that BN-$ 19^{T} $ represents a novel species in the genus Rhizobium. The DNA G + C content was 60.1 mol%. The dominant fatty acids of strain BN-$ 19^{T} $ were $ C_{19:0} $ cyclo ω8c, summed feature 2 ($ C_{14:0} $ 3OH and/or $ C_{16:1} $ iso I) and summed feature 8 ($ C_{18:1} $ω7c). Some phenotypic features also differentiate the strain BN-$ 19^{T} $ from the related species. On the basis of these results, strain BN-$ 19^{T} $ is considered to represent a novel species in the genus Rhizobium, for which the name Rhizobium pakistanensis sp. nov. is proposed. The type strain is BN-$ 19^{T} $ (=LMG $ 27895^{T} $ = CCBAU $ 101086^{T} $). | ||
| 650 | 4 | |a Rhizobia |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Housekeeping genes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Polyphasic taxonomy |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhang, Yu Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Ali, Safdar |e verfasserin |4 aut | |
| 700 | 1 | |a Sui, Xin Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiao Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Amara, Ummay |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wen Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Hayat, Rifat |e verfasserin |4 aut | |
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