Extraction of aflatoxins by using mesoporous silica (type UVM-7), and their quantitation by HPLC-MS
Abstract A solid-phase extraction procedure has been developed by using a sorbent derived from UVM-7 mesoporous silica. The sorbent was applied to the extraction of aflatoxins $ B_{1} $, $ B_{2} $, $ G_{1} $ and $ G_{2} $ from tea samples followed by HPLC with mass spectrometric detection. The sorbent was characterized by transmission electron microscopy, nuclear magnetic resonance, X-ray diffraction and nitrogen adsorption-desorption. UVM-7 is found to be the best solid phase. The amount of solid-phase, type and volume of eluent, pH value and ionic strength and breakthrough volume were optimized. Following the recommended procedure, recoveries between 96.0 and 98.2% were achieved, with RSD values of <5.1%, and the limits of detection are in the range from 0.14 to 0.7 μg·$ kg^{−1} $. The material is reusable. The method was applied to the analysis of real tea samples. A low matrix effect is found, and recoveries are >88%. The results were compared with those obtained by immunoaffinity columns as a reference method. Only low concentrations of aflatoxin $ G_{2} $ were found in some samples, and results obtained with both methods are shown to be statistically sound and comparable. Graphical abstractSchematic representation of a mesoporous silica sorbent (type UVM-7) for the extraction of aflatoxins (AF) from tea by solid-phase extraction (SPE), and its determination by liquid chromatography. The morphology of the material allows to retain the analytes very well..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:186 |
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| Enthalten in: |
Microchimica acta - 186(2019), 12 vom: 16. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pellicer-Castell, Enric [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
Food analysis |
| doi: |
10.1007/s00604-019-3958-8 |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract A solid-phase extraction procedure has been developed by using a sorbent derived from UVM-7 mesoporous silica. The sorbent was applied to the extraction of aflatoxins $ B_{1} $, $ B_{2} $, $ G_{1} $ and $ G_{2} $ from tea samples followed by HPLC with mass spectrometric detection. The sorbent was characterized by transmission electron microscopy, nuclear magnetic resonance, X-ray diffraction and nitrogen adsorption-desorption. UVM-7 is found to be the best solid phase. The amount of solid-phase, type and volume of eluent, pH value and ionic strength and breakthrough volume were optimized. Following the recommended procedure, recoveries between 96.0 and 98.2% were achieved, with RSD values of <5.1%, and the limits of detection are in the range from 0.14 to 0.7 μg·$ kg^{−1} $. The material is reusable. The method was applied to the analysis of real tea samples. A low matrix effect is found, and recoveries are >88%. The results were compared with those obtained by immunoaffinity columns as a reference method. Only low concentrations of aflatoxin $ G_{2} $ were found in some samples, and results obtained with both methods are shown to be statistically sound and comparable. Graphical abstractSchematic representation of a mesoporous silica sorbent (type UVM-7) for the extraction of aflatoxins (AF) from tea by solid-phase extraction (SPE), and its determination by liquid chromatography. The morphology of the material allows to retain the analytes very well. | ||
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| 650 | 4 | |a Mycotoxin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Materials |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a Solid-phase extraction |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nanomaterials |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hierarchical porosity |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Belenguer-Sapiña, Carolina |e verfasserin |4 aut | |
| 700 | 1 | |a Borràs, Vicent J. |e verfasserin |4 aut | |
| 700 | 1 | |a Amorós, Pedro |e verfasserin |4 aut | |
| 700 | 1 | |a El Haskouri, Jamal |e verfasserin |4 aut | |
| 700 | 1 | |a Herrero-Martínez, José Manuel |e verfasserin |4 aut | |
| 700 | 1 | |a Mauri-Aucejo, Adela R. |e verfasserin |4 aut | |
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