The mushroom ribosome-inactivating protein lyophyllin exerts deleterious effects on mouse embryonic development in vitro
Abstract Earlier investigations disclose that some plant ribosome-inactivating proteins (RIPs) adversely affect mouse embryonic development. In the present study, a mushroom RIP, namely lyophyllin from Lyophyllum shimeji, was isolated, partially sequenced, and its translation inhibitory activity determined. Its teratogenicity was studied by using a technique entailing microinjection and postimplantation whole-embryo culture. It was found that embryonic abnormalities during the period of organogenesis from E8.5 to E9.5 were induced by lyophyllin at a concentration as low as 50 μg/ml, and when the lyophyllin concentration was raised, the number of abnormal embryos increased, the final somite number decreased, and the abnormalities increased in severity. The affected embryonic structures included the cranial neural tube, forelimb buds, branchial arches, and body axis, while optic and otic placodes were more resistant. Lyophyllin at a concentration higher than 500 μg/ml also induced forebrain blisters within the cranial mesenchyme. When the abnormal embryos were examined histologically, an increase of cell death was found to be associated with abnormal structures, indicating that cell death may be one of the underlying causes of teratogenicity of the mushroom RIP. This constitutes the first report on the teratogenicity of a mushroom RIP..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:85 |
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| Enthalten in: |
Applied microbiology and biotechnology - 85(2009), 4 vom: 01. Juli, Seite 985-993 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chan, W. Y. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Hypsin |
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| Anmerkungen: |
© Springer-Verlag 2009 |
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| doi: |
10.1007/s00253-009-2048-y |
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SPR002960680 |
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| 520 | |a Abstract Earlier investigations disclose that some plant ribosome-inactivating proteins (RIPs) adversely affect mouse embryonic development. In the present study, a mushroom RIP, namely lyophyllin from Lyophyllum shimeji, was isolated, partially sequenced, and its translation inhibitory activity determined. Its teratogenicity was studied by using a technique entailing microinjection and postimplantation whole-embryo culture. It was found that embryonic abnormalities during the period of organogenesis from E8.5 to E9.5 were induced by lyophyllin at a concentration as low as 50 μg/ml, and when the lyophyllin concentration was raised, the number of abnormal embryos increased, the final somite number decreased, and the abnormalities increased in severity. The affected embryonic structures included the cranial neural tube, forelimb buds, branchial arches, and body axis, while optic and otic placodes were more resistant. Lyophyllin at a concentration higher than 500 μg/ml also induced forebrain blisters within the cranial mesenchyme. When the abnormal embryos were examined histologically, an increase of cell death was found to be associated with abnormal structures, indicating that cell death may be one of the underlying causes of teratogenicity of the mushroom RIP. This constitutes the first report on the teratogenicity of a mushroom RIP. | ||
| 650 | 4 | |a Ribosome-inactivating proteins |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Teratogenic effects |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Mouse postimplantation embryos |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a Velutin |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Ng, T. B. |4 aut | |
| 700 | 1 | |a Lam, Joyce S. Y. |4 aut | |
| 700 | 1 | |a Wong, Jack H. |4 aut | |
| 700 | 1 | |a Chu, K. T. |4 aut | |
| 700 | 1 | |a Ngai, P. H. K. |4 aut | |
| 700 | 1 | |a Lam, S. K. |4 aut | |
| 700 | 1 | |a Wang, H. X. |4 aut | |
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