An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host–guest recognition among phosphate pillar[5]arenepalladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue
T4 polynucleotide kinase (T4 PNK) helps with DNA recombination and repair. In this work, a phosphate pillar[5]arenepalladium nanoparticles@reduced graphene oxide nanocomposite (PP5@PdNPs@rGO)–based electrochemical biosensor was created to identify T4 PNK activities. The PP5 used to complex toluidine blue (TB) guest molecules is water-soluble. With T4 PNK and ATP, the substrate DNA, which included a 5ʹ-hydroxyl group, initially self-assembled over the gold electrode surface by chemical adsorption of the thiol units. Strong phosphate-$ Zr^{4+} $-phosphate chemistry allowed $ Zr^{4+} $ to act as a bridge between phosphorylated DNA and PP5@PdNPs@rGO. Through a supramolecular host–guest recognition connection, TB molecules were able to penetrate the PP5 cavity, where they produced a stronger electrochemical response. With a 5 × $ 10^{−7} $ U $ mL^{−1} $ detection limit, the electrochemical signal is linear in the $ 10^{−6} $ to 1 U $ mL^{−1} $ T4 PNK concentration range. It was also effective in measuring HeLa cell lysate–related PNK activities and screening PNK inhibitors. Nucleotide kinase–target drug development, clinical diagnostics, and screening for inhibitors all stand to benefit greatly from the suggested technology, which offers a unique sensing mechanism for kinase activity measurement. Graphical Abstract.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:190 |
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| Enthalten in: |
Microchimica acta ... - 190(2023), 10 vom: 16. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Yongju [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
Electrochemical biosensors |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s00604-023-05983-w |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 3 | |a An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host–guest recognition among phosphate pillar[5]arenepalladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue |
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| 520 | |a T4 polynucleotide kinase (T4 PNK) helps with DNA recombination and repair. In this work, a phosphate pillar[5]arenepalladium nanoparticles@reduced graphene oxide nanocomposite (PP5@PdNPs@rGO)–based electrochemical biosensor was created to identify T4 PNK activities. The PP5 used to complex toluidine blue (TB) guest molecules is water-soluble. With T4 PNK and ATP, the substrate DNA, which included a 5ʹ-hydroxyl group, initially self-assembled over the gold electrode surface by chemical adsorption of the thiol units. Strong phosphate-$ Zr^{4+} $-phosphate chemistry allowed $ Zr^{4+} $ to act as a bridge between phosphorylated DNA and PP5@PdNPs@rGO. Through a supramolecular host–guest recognition connection, TB molecules were able to penetrate the PP5 cavity, where they produced a stronger electrochemical response. With a 5 × $ 10^{−7} $ U $ mL^{−1} $ detection limit, the electrochemical signal is linear in the $ 10^{−6} $ to 1 U $ mL^{−1} $ T4 PNK concentration range. It was also effective in measuring HeLa cell lysate–related PNK activities and screening PNK inhibitors. Nucleotide kinase–target drug development, clinical diagnostics, and screening for inhibitors all stand to benefit greatly from the suggested technology, which offers a unique sensing mechanism for kinase activity measurement. Graphical Abstract | ||
| 650 | 4 | |a Host–guest recognition | |
| 650 | 4 | |a Phosphate pillar[5]arene | |
| 650 | 4 | |a Palladium nanoparticles | |
| 650 | 4 | |a Toluidine blue | |
| 650 | 4 | |a Electrochemical biosensors | |
| 650 | 4 | |a Gold electrode modification | |
| 650 | 4 | |a T4 polynucleotide kinase | |
| 700 | 1 | |a Yi, Jinfei |4 aut | |
| 700 | 1 | |a Su, Aiwen |4 aut | |
| 700 | 1 | |a Zhang, Yanli |4 aut | |
| 700 | 1 | |a Wang, Hongbin |4 aut | |
| 700 | 1 | |a Yang, Lijuan |4 aut | |
| 700 | 1 | |a Yang, Wenrong |4 aut | |
| 700 | 1 | |a Pang, Pengfei |4 aut | |
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