Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats
Abstract Arsenic being a toxic metalloid ubiquitously persists in environment and causes several health complications including female reproductive anomalies. Epidemiological studies documented birth anomalies due to arsenic exposure. Augmented reactive oxygen species (ROS) generation and quenched antioxidant pool are foremost consequences of arsenic threat. On the contrary, Vitamin E (VE) and C (VC) are persuasive antioxidants and conventionally used in toxicity management. Present study was designed to explore the extent of efficacy of combined VE and VC (VEC) against Sodium arsenite ($ NaAsO_{2} $) mediated ovarian damage. Thirty-six female Wistar rats were randomly divided into three groups (Grs) and treated for consecutive 30 days; Gr I (control) was vehicle fed, Gr II (treated) was gavaged with $ NaAsO_{2 } $(3 mg/kg/day), Gr III (supplement) was provided with VE (400 mg/kg/day) & VC (200 mg/kg/day) along with $ NaAsO_{2} $. Marked histological alterations were evidenced by disorganization in oocyte, granulosa cells and zona pellucida layers in treated group. Considerable reduction of different growing follicles along with increased atretic follicles was noted in treated group. Altered activities $ ofΔ^{5} $ 3β-Hydroxysteroid dehydrogenase and 17β-Hydroxysteroid dehydrogenase accompanied by reduced luteinizing hormone, follicle-stimulating hormone and estradiol levels were observed in treated animals. Irregular estrous cyclicity pattern was also observed due to $ NaAsO_{2} $ threat. Surplus ROS production affected ovarian antioxidant strata as evidenced by altered oxidative stress markers. Provoked oxidative strain further affects DNA status of ovary. However, supplementation with VEC caused notable restoration from such disparaging effects of $ NaAsO_{2} $ toxicities. Antioxidant and antiapoptotic attributes of those vitamins might be liable for such restoration..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:396 |
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| Enthalten in: |
Naunyn-Schmiedeberg's archives of pharmacology - 396(2023), 10 vom: 02. Mai, Seite 2701-2720 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mondal, Rubia [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s00210-023-02491-9 |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Arsenic being a toxic metalloid ubiquitously persists in environment and causes several health complications including female reproductive anomalies. Epidemiological studies documented birth anomalies due to arsenic exposure. Augmented reactive oxygen species (ROS) generation and quenched antioxidant pool are foremost consequences of arsenic threat. On the contrary, Vitamin E (VE) and C (VC) are persuasive antioxidants and conventionally used in toxicity management. Present study was designed to explore the extent of efficacy of combined VE and VC (VEC) against Sodium arsenite ($ NaAsO_{2} $) mediated ovarian damage. Thirty-six female Wistar rats were randomly divided into three groups (Grs) and treated for consecutive 30 days; Gr I (control) was vehicle fed, Gr II (treated) was gavaged with $ NaAsO_{2 } $(3 mg/kg/day), Gr III (supplement) was provided with VE (400 mg/kg/day) & VC (200 mg/kg/day) along with $ NaAsO_{2} $. Marked histological alterations were evidenced by disorganization in oocyte, granulosa cells and zona pellucida layers in treated group. Considerable reduction of different growing follicles along with increased atretic follicles was noted in treated group. Altered activities $ ofΔ^{5} $ 3β-Hydroxysteroid dehydrogenase and 17β-Hydroxysteroid dehydrogenase accompanied by reduced luteinizing hormone, follicle-stimulating hormone and estradiol levels were observed in treated animals. Irregular estrous cyclicity pattern was also observed due to $ NaAsO_{2} $ threat. Surplus ROS production affected ovarian antioxidant strata as evidenced by altered oxidative stress markers. Provoked oxidative strain further affects DNA status of ovary. However, supplementation with VEC caused notable restoration from such disparaging effects of $ NaAsO_{2} $ toxicities. Antioxidant and antiapoptotic attributes of those vitamins might be liable for such restoration. | ||
| 650 | 4 | |a Sodium arsenite | |
| 650 | 4 | |a DNA damage | |
| 650 | 4 | |a Granulosa cell | |
| 650 | 4 | |a Vitamin E | |
| 650 | 4 | |a Vitamin C | |
| 700 | 1 | |a Pal, Priyankar |4 aut | |
| 700 | 1 | |a Biswas, Sagnik |4 aut | |
| 700 | 1 | |a Chattopadhyay, Alok |4 aut | |
| 700 | 1 | |a Bandyopadhyay, Amit |4 aut | |
| 700 | 1 | |a Mukhopadhyay, Aparna |4 aut | |
| 700 | 1 | |a Mukhopadhyay, Prabir Kumar |0 (orcid)0000-0003-3190-2915 |4 aut | |
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