Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics

Abstract Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered..

Medienart:

E-Artikel

Erscheinungsjahr:

2023

Erschienen:

2023

Enthalten in:

Zur Gesamtaufnahme - volume:27

Enthalten in:

Microfluidics and nanofluidics - 27(2023), 10 vom: 11. Sept.

Sprache:

Englisch

Beteiligte Personen:

İnce, Gamze Tilbe [VerfasserIn]
Yüksekkaya, Mehmet [VerfasserIn]
Haberal, Orhan Erdem [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

Themen:

COVID-19
DNA amplification
MEMS
Microfluidics
Point of care testing
Polymerase chain reaction

Anmerkungen:

© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:

10.1007/s10404-023-02677-w

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2145495703

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