Calcium Supplementation on Glucose Tolerance, Oxidative Stress, and Reproductive Outcomes of Diabetic Rats and Their Offspring
Abstract Diabetes mellitus increases the risk of obstetric complications, morbidity, and infant mortality. Controlled nutritional therapy with micronutrients has been employed. However, the effect of calcium ($ Ca^{2+} $) supplementation on diabetic pregnancy is unclear. We aimed to evaluate whether diabetic rats supplemented with $ Ca^{2+} $ during pregnancy present better glucose tolerance, redox status, embryonic and fetal development, newborn weight, and the prooxidant and antioxidant balance of male and female pups. For this, newborn rats received the beta-cytotoxic drug streptozotocin for inducing diabetes on the day of birth. In adulthood, these rats were mated and treated with $ Ca^{2+} $ twice a day from day 0 to day 20 of pregnancy. On day 17, the pregnant rats were submitted to the oral glucose tolerance test (OGTT). At the end of pregnancy, they were anesthetized and killed to collect blood and pancreas samples. The uterine horns were exposed for an evaluation of maternal reproductive outcomes and embryofetal development, and the offspring’s liver samples were collected for redox status measurement. Nondiabetic and diabetic rats supplemented with $ Ca^{2+} $ showed no influence on glucose tolerance, redox status, insulin synthesis, serum calcium levels, and embryofetal losses. The reduced rate of newborns classified as adequate for gestational age (AGA) and higher rates of LGA (large) and small (LGA) newborns and higher -SH and GSH-Px antioxidant activities in female pups were observed in diabetic dams, regardless of supplementation. Thus, maternal supplementation caused no improvement in glucose tolerance, oxidative stress biomarkers, embryofetal growth and development, and antioxidants in pups from diabetic mothers..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Reproductive sciences - 30(2023), 9 vom: 31. März, Seite 2813-2828 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Klöppel, Eduardo [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Society for Reproductive Investigation 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s43032-023-01217-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Diabetes mellitus increases the risk of obstetric complications, morbidity, and infant mortality. Controlled nutritional therapy with micronutrients has been employed. However, the effect of calcium ($ Ca^{2+} $) supplementation on diabetic pregnancy is unclear. We aimed to evaluate whether diabetic rats supplemented with $ Ca^{2+} $ during pregnancy present better glucose tolerance, redox status, embryonic and fetal development, newborn weight, and the prooxidant and antioxidant balance of male and female pups. For this, newborn rats received the beta-cytotoxic drug streptozotocin for inducing diabetes on the day of birth. In adulthood, these rats were mated and treated with $ Ca^{2+} $ twice a day from day 0 to day 20 of pregnancy. On day 17, the pregnant rats were submitted to the oral glucose tolerance test (OGTT). At the end of pregnancy, they were anesthetized and killed to collect blood and pancreas samples. The uterine horns were exposed for an evaluation of maternal reproductive outcomes and embryofetal development, and the offspring’s liver samples were collected for redox status measurement. Nondiabetic and diabetic rats supplemented with $ Ca^{2+} $ showed no influence on glucose tolerance, redox status, insulin synthesis, serum calcium levels, and embryofetal losses. The reduced rate of newborns classified as adequate for gestational age (AGA) and higher rates of LGA (large) and small (LGA) newborns and higher -SH and GSH-Px antioxidant activities in female pups were observed in diabetic dams, regardless of supplementation. Thus, maternal supplementation caused no improvement in glucose tolerance, oxidative stress biomarkers, embryofetal growth and development, and antioxidants in pups from diabetic mothers. | ||
| 650 | 4 | |a Hyperglycemia | |
| 650 | 4 | |a Rodent | |
| 650 | 4 | |a Pregnancy | |
| 650 | 4 | |a Calcium | |
| 650 | 4 | |a Redox status | |
| 700 | 1 | |a Souza, Maysa Rocha |0 (orcid)0000-0002-2144-5004 |4 aut | |
| 700 | 1 | |a Barco, Vinícius Soares |0 (orcid)0000-0003-1759-0011 |4 aut | |
| 700 | 1 | |a Gallego, Franciane Quintanilha |0 (orcid)0000-0002-6081-7763 |4 aut | |
| 700 | 1 | |a Sinzato, Yuri Karen |0 (orcid)0000-0002-2973-1099 |4 aut | |
| 700 | 1 | |a Corrente, José Eduardo |0 (orcid)0000-0001-5478-4996 |4 aut | |
| 700 | 1 | |a Rodrigues, Tiago |0 (orcid)0000-0003-1277-0224 |4 aut | |
| 700 | 1 | |a Volpato, Gustavo Tadeu |0 (orcid)0000-0002-4753-3264 |4 aut | |
| 700 | 1 | |a Damasceno, Débora Cristina |0 (orcid)0000-0002-7003-9643 |4 aut | |
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