A Novel Multiplex RT-PCR for Simultaneous Detection of Malaria, Chikungunya and Dengue Infection (MCD-RT-PCR)
Abstract The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single-step multiplex RT-PCR assay using in-house designed primers which could detect as low as 0.25 ng/µl of isolated RNA. The assay was evaluated on 104 samples for the detection of these three pathogens. The sensitivity of the assay was found to be 100% for detecting all three pathogens (P < 0.0001), whereas the specificity was 100, 97 and 93% for Plasmodium, dengue and chikungunya, respectively. This novel multiplex RT-PCR assay is a prompt, robust, accurate and cost effective that could detect all three pathogens simultaneously which ensures an accurate diagnosis and better clinical management..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences / A - 93(2023), 3 vom: 28. Apr., Seite 755-761 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sinha, Swati [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© The Author(s), under exclusive licence to The National Academy of Sciences, India 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s40011-023-01447-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2145319263 |
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| 520 | |a Abstract The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single-step multiplex RT-PCR assay using in-house designed primers which could detect as low as 0.25 ng/µl of isolated RNA. The assay was evaluated on 104 samples for the detection of these three pathogens. The sensitivity of the assay was found to be 100% for detecting all three pathogens (P < 0.0001), whereas the specificity was 100, 97 and 93% for Plasmodium, dengue and chikungunya, respectively. This novel multiplex RT-PCR assay is a prompt, robust, accurate and cost effective that could detect all three pathogens simultaneously which ensures an accurate diagnosis and better clinical management. | ||
| 650 | 4 | |a Malaria | |
| 650 | 4 | |a Dengue | |
| 650 | 4 | |a Chikungunya | |
| 650 | 4 | |a Co-infection | |
| 650 | 4 | |a Multiplex RT-PCR | |
| 700 | 1 | |a Gahtori, Renuka |4 aut | |
| 700 | 1 | |a Kumari, Preeti |4 aut | |
| 700 | 1 | |a Baharia, Rajendra K. |4 aut | |
| 700 | 1 | |a Saxena, Mritunjay |4 aut | |
| 700 | 1 | |a Pande, Veena |4 aut | |
| 700 | 1 | |a Anvikar, Anupkumar R. |4 aut | |
| 700 | 1 | |a Savargaonkar, Deepali |4 aut | |
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