Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products
Abstract Fluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the C5 position of the pyrimidine base through a trans-alkene spacer. The substrate efficiency of the labeled nucleotides has been tested in recombinase polymerase amplification (RPA). As a result of RPA, full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) have been formed with a high density of fluorescent label..
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
---|---|
Enthalten in: |
Russian journal of bioorganic chemistry - 49(2023), 5 vom: 21. Aug., Seite 1151-1158 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Shershov, V. E. [VerfasserIn] |
---|
Links: |
Volltext [lizenzpflichtig] |
---|
Anmerkungen: |
© Pleiades Publishing, Ltd. 2023 |
---|
doi: |
10.1134/S1068162023050242 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
OLC2145128409 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | OLC2145128409 | ||
003 | DE-627 | ||
005 | 20240118102904.0 | ||
007 | tu | ||
008 | 240118s2023 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1134/S1068162023050242 |2 doi | |
035 | |a (DE-627)OLC2145128409 | ||
035 | |a (DE-He213)S1068162023050242-p | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 540 |q VZ |
100 | 1 | |a Shershov, V. E. |e verfasserin |4 aut | |
245 | 1 | 0 | |a Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a © Pleiades Publishing, Ltd. 2023 | ||
520 | |a Abstract Fluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the C5 position of the pyrimidine base through a trans-alkene spacer. The substrate efficiency of the labeled nucleotides has been tested in recombinase polymerase amplification (RPA). As a result of RPA, full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) have been formed with a high density of fluorescent label. | ||
700 | 1 | |a Lapa, S. A. |4 aut | |
700 | 1 | |a Levashova, A. I. |4 aut | |
700 | 1 | |a Shishkin, I. Yu. |4 aut | |
700 | 1 | |a Shtylev, G. F. |4 aut | |
700 | 1 | |a Shekalova, E. Yu. |4 aut | |
700 | 1 | |a Vasiliskov, V. A. |4 aut | |
700 | 1 | |a Zasedatelev, A. S. |4 aut | |
700 | 1 | |a Kuznetsova, V. E. |4 aut | |
700 | 1 | |a Chudinov, A. V. |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Russian journal of bioorganic chemistry |d Pleiades Publishing, 1993 |g 49(2023), 5 vom: 21. Aug., Seite 1151-1158 |w (DE-627)171227131 |w (DE-600)1167762-4 |w (DE-576)038720973 |x 1068-1620 |7 nnns |
773 | 1 | 8 | |g volume:49 |g year:2023 |g number:5 |g day:21 |g month:08 |g pages:1151-1158 |
856 | 4 | 1 | |u https://doi.org/10.1134/S1068162023050242 |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
951 | |a AR | ||
952 | |d 49 |j 2023 |e 5 |b 21 |c 08 |h 1151-1158 |