Semiautomatic assessment of endothelial density and morphology in organ-cultured corneas — potential predictors for transplantation suitability and clinical outcome?

Background The quality of the endothelial cell layer is a major criterion for the approval of organ-cultured human donor-corneas for transplantation. We wanted to compare the predictive capacities of initial endothelial density and endothelium cell morphology for the approval of donor corneas for transplantation and for the clinical outcome after transplantation. Methods The endothelial density and endothelium morphology in organ culture were examined by semiautomatic assessment of 1031 donor corneas. We performed a statistical analysis for correlations of donor-data and cultivation parameters regarding their predictive capacities for the final approval of donor corneas for transplantation and the clinical outcome of 202 transplanted patients. Results Corneal endothelium cell density proved to be the only parameter with a certain predictive capacity with regard to the final decision, whether donor corneas are suitable for transplantation — however, the correlation was low (area under the curve [AUC] = 0.655). Endothelial cell morphology lacked any predictive power (AUC = 0.597). The clinical outcome regarding visual acuity seemed to be largely independent from both corneal endothelial cell density and morphology. Sub-analyses on transplanted patients stratified for their diagnoses vindicated these findings. Conclusions Higher endothelial density (above a cut-off level of 2000 cells/$ mm^{2} $), as well as better endothelial morphology do not seem to be critical for transplant-corneal functionality in organ culture and up to 2 years after transplantation. Comparable long-term studies on graft survival are recommended to determine, whether the present endothelial density cut-off levels might be too stringent..

Medienart:

E-Artikel

Erscheinungsjahr:

2023

Erschienen:

2023

Enthalten in:

Zur Gesamtaufnahme - volume:261

Enthalten in:

Graefe's archive for clinical and experimental ophthalmology - 261(2023), 9 vom: 28. Apr., Seite 2593-2602

Sprache:

Englisch

Beteiligte Personen:

Filev, Filip [VerfasserIn]
Stein, Mathias [VerfasserIn]
Schultheiss, Maximilian [VerfasserIn]
Fitzek, Antonia D. E. [VerfasserIn]
Feuerstake, Jana [VerfasserIn]
Engel, Oliver [VerfasserIn]
Hellwinkel, Olaf J. C. [VerfasserIn]

Links:

Volltext [kostenfrei]

Themen:

Cell density
Clinical outcome
Corneal endothelium
Morphology

RVK:

RVK Klassifikation

Anmerkungen:

© The Author(s) 2023

doi:

10.1007/s00417-023-06079-0

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2145040110

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