Details der Publikation - Diagnostic genosensor for detection of rotavirus based on HFGNs/MXene/PPY signal amplification

Diagnostic genosensor for detection of rotavirus based on HFGNs/MXene/PPY signal amplification

A novel genosensor was developed for rotavirus specific cDNA sequence detection. The genosensor was comprised of hierarchical flower-like gold nanostructures, MXene, and polypyrrole (HFGNs/MXene/PPY) nanocomposite as a signal amplification tag, specific antisense ssDNA oligonucleotide as a recognition bioelement, and methylene blue (MB) as a redox marker. The morphological and electrochemical features of the biosensor were first tested and optimized and the high performance of the platform was confirmed in terms of sensitivity and reproducibility. Then, 20 rotavirus RNA isolated from clinical and cell-cultured samples (10 positive and 10 negative confirmed by RT-PCR and electrophoresis methods) were evaluated by the genosensor. The analysis results revealed that the genosensor is able to differentiate successfully between the positive and negative control groups. The developed genosensor for rotavirus RNA detection presented an excellent limit of detection of ∼ 0.8 aM and a determination range of $ 10^{−18} $ and $ 10^{−7} $ M. In addition, the ssDNA/HFGNs/MXene/PPY/GCE showed high selectivity and long-term stability of ~ 24 days. Therefore, this novel genosensor would be of great benefit for the clinical diagnosis of rotavirus. Graphical abstract.

Medienart:	E-Artikel

Erscheinungsjahr:	2023
Erschienen:	2023

Enthalten in:	Zur Gesamtaufnahme - volume:190
Enthalten in:	Microchimica acta - 190(2023), 8 vom: 17. Juli

Sprache:	Englisch

Beteiligte Personen:	Ketabi, Kiana [VerfasserIn] Soleimanjahi, Hoorieh [VerfasserIn] Teimoori, Ali [VerfasserIn] Hatamluyi, Behnaz [VerfasserIn] Rezayi, Majid [VerfasserIn] Meshkat, Zahra [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Biosensing techniques DNA biosensor Differential pulse voltammetry Electrochemical genosensor Nanocomposites Virus diagnosis

Anmerkungen:	© The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:	10.1007/s00604-023-05871-3

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2144501726

Internformat


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520			\|a A novel genosensor was developed for rotavirus specific cDNA sequence detection. The genosensor was comprised of hierarchical flower-like gold nanostructures, MXene, and polypyrrole (HFGNs/MXene/PPY) nanocomposite as a signal amplification tag, specific antisense ssDNA oligonucleotide as a recognition bioelement, and methylene blue (MB) as a redox marker. The morphological and electrochemical features of the biosensor were first tested and optimized and the high performance of the platform was confirmed in terms of sensitivity and reproducibility. Then, 20 rotavirus RNA isolated from clinical and cell-cultured samples (10 positive and 10 negative confirmed by RT-PCR and electrophoresis methods) were evaluated by the genosensor. The analysis results revealed that the genosensor is able to differentiate successfully between the positive and negative control groups. The developed genosensor for rotavirus RNA detection presented an excellent limit of detection of ∼ 0.8 aM and a determination range of $ 10^{−18} $ and $ 10^{−7} $ M. In addition, the ssDNA/HFGNs/MXene/PPY/GCE showed high selectivity and long-term stability of ~ 24 days. Therefore, this novel genosensor would be of great benefit for the clinical diagnosis of rotavirus. Graphical abstract
650		4	\|a Virus diagnosis
650		4	\|a Electrochemical genosensor
650		4	\|a Differential pulse voltammetry
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700	1		\|a Rezayi, Majid \|4 aut
700	1		\|a Meshkat, Zahra \|4 aut
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912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
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912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
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912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
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912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
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912			\|a GBV_ILN_2055
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912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
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912			\|a GBV_ILN_2119
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912			\|a GBV_ILN_4125
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912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
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Diagnostic genosensor for detection of rotavirus based on HFGNs/MXene/PPY signal amplification

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