Multi-parameter surface plasmon resonance instrument for multiple nucleic acid quantitative detection
Multiplex nucleic acid assays can simultaneously detect the characteristics of different target nucleic acids in complex mixtures and are used in disease diagnosis, environmental monitoring, and food safety. However, traditional nucleic acid amplification assays have limitations such as complicated operation, long detection time, unstable fluorescent labeling, and mutual interference of multiplex nucleic acids. We developed a real-time, rapid, and label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection. The multiparametric optical system based on total internal reflection solves the multiplex detection problem by cooperating with linear light source, prism, photodetector, and mechanical transmission system. An adaptive threshold consistency correction algorithm is proposed to solve the problem of inconsistent responsiveness of different detection channels and the inability of quantitative comparison. The instrument achieves label-free and amplification-free rapid detection of these biomarkers for miRNA-21 and miRNA-141, which are widely expressed in breast cancer and prostate cancer. The multiplex nucleic acid detection takes 30 min and the biosensor has good repeatability and specificity. The instrument has a limit of detection (LODs) of 50 nM for target oligonucleotides, and the smallest absolute amount of sample that can be detected is about 4 pmol. It provides a simple and efficient point-of-care testing (POCT) detection platform for small molecules such as DNA and miRNA. Graphical abstract.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Biomedical microdevices - 25(2023), 3 vom: 07. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Huixiang [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Biosensor |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s10544-023-00664-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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OLC2144355290 |
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| 520 | |a Multiplex nucleic acid assays can simultaneously detect the characteristics of different target nucleic acids in complex mixtures and are used in disease diagnosis, environmental monitoring, and food safety. However, traditional nucleic acid amplification assays have limitations such as complicated operation, long detection time, unstable fluorescent labeling, and mutual interference of multiplex nucleic acids. We developed a real-time, rapid, and label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection. The multiparametric optical system based on total internal reflection solves the multiplex detection problem by cooperating with linear light source, prism, photodetector, and mechanical transmission system. An adaptive threshold consistency correction algorithm is proposed to solve the problem of inconsistent responsiveness of different detection channels and the inability of quantitative comparison. The instrument achieves label-free and amplification-free rapid detection of these biomarkers for miRNA-21 and miRNA-141, which are widely expressed in breast cancer and prostate cancer. The multiplex nucleic acid detection takes 30 min and the biosensor has good repeatability and specificity. The instrument has a limit of detection (LODs) of 50 nM for target oligonucleotides, and the smallest absolute amount of sample that can be detected is about 4 pmol. It provides a simple and efficient point-of-care testing (POCT) detection platform for small molecules such as DNA and miRNA. Graphical abstract | ||
| 650 | 4 | |a Biosensor | |
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| 650 | 4 | |a Nucleic acid hybridization | |
| 650 | 4 | |a Surface plasmon resonance (SPR) | |
| 700 | 1 | |a Wang, Honggang |4 aut | |
| 700 | 1 | |a Huang, Yafeng |4 aut | |
| 700 | 1 | |a Zhang, Hao |4 aut | |
| 700 | 1 | |a Fu, Yongdong |4 aut | |
| 700 | 1 | |a Yang, Zhenwei |4 aut | |
| 700 | 1 | |a Chen, Yuanyuan |4 aut | |
| 700 | 1 | |a Qiu, Xianbo |4 aut | |
| 700 | 1 | |a Yu, Duli |4 aut | |
| 700 | 1 | |a Zhang, Lulu |0 (orcid)0000-0003-2437-1546 |4 aut | |
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