The bioreaction and immune responses of PEG-coated silica NPs and the role of the surface density coating after oral administration into mice

Abstract Silica nanoparticles (SiNPs) have many physical and chemical characteristics that make it useful tools for different medical and biological applications. However, toxicities and biodistribution of SiNPs after in vivo administration need further investigation, when these materials are used as drug delivery systems (DDS). In this study, surface coating of SiNPs with different densities of polyethylene glycol (PEG) could control their biodistribution and in vivo interaction, especially in different tissue such as blood, tumor, spleen, and liver. In this study, after oral administration, the higher coating density of PEG on the surface of SiNPs ($ SiNPs_PEG_{H} $ system) showed prolonged circulation time in the blood compared with lower surface coating density of PEG ($ SiNPs_PEG_{L} $ system) or amine surface coating SiNPs ($ SiNPs_NH_{2} $ system). Quantitatively, the $ SiNPs_NH_{2} $ system exhibited 3.5% (dose/ml of serum) after 24 h of the oral administration. While $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems showed higher blood retention with 8.3% and 12.6% (dose/ml of serum), respectively. As a result, the accumulation ratio of SiNPs systems in the tumor showed 2.4%, 4.4% and 6% (dose/ml of serum) for $ SiNPs_NH_{2} $, $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems, respectively. Both $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems showed lower accumulation profiles in the healthy organs such as the liver (6.9% and 6.3% (of dose/gm), respectively), spleen (11.5% and 11% (of dose/gm), respectively), and Peyer’s patches (4.7% and 3.2% (of dose/gm), respectively) compared with $ SiNPs_NH_{2} $ system. Interestingly, the PEG or amine group surface coating could control the biodistribution profiles in tumor site as well as healthy organs differently. Additionally, $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems differently induced immune responses based on the PEG surface coating density after oral administration. The anti-PEG immunoglobulin (Ig) M and total IgM antibodies were induced by $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems while the $ SiNPs_NH_{2} $ system induced total IgM antibody only. Quantitatively, the induction level of IgM antibody of $ SiNPs_NH_{2} $, $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems were 0.82, 0.56 and 0.45 times higher compared to IgM antibody induced by bovine serums albumin (BSA) as a positive control. While the level anti-PEG IgM antibody of $ SiNPs_PEG_{L} $ and $ SiNPs_PEG_{H} $ systems were 10 and 13.3 times higher compared with that of the $ SiNPs_NH_{2} $ system. Generally, PEG surface coating plays an important role to control the SiNPs-based DDS biodistribution and its immunogenicity. Furthermore, the obtained data from this study can improve the use of SiNPs as DDS for tumor targeting with lower immunogenicity and effective accumulation in the tumor site..

Medienart:	Artikel

Erscheinungsjahr:	2023
Erschienen:	2023

Enthalten in:	Zur Gesamtaufnahme - volume:13
Enthalten in:	Applied Nanoscience - 13(2023), 8 vom: 27. Jan., Seite 5563-5578

Sprache:	Englisch

Beteiligte Personen:	Abu-Dief, Ahmed M. [VerfasserIn] Alsehli, Mosa [VerfasserIn] Awaad, Aziz [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Immunogenicity Liver Polyethylene glycol Silica nanoparticles Spleen Surface density coating Tumor

Anmerkungen:	© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:	10.1007/s13204-023-02770-0

funding:
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PPN (Katalog-ID):	OLC214417850X