Biorefinery system for production of thermostable exopolysaccharide by a novel thermophile Brevibacillus borstelensis MK878423 and its study on impact of glucose utilization
Abstract Microbial exopolysaccharides, especially from thermophiles, are gaining attention due to their thermostability and reduced viscosity at elevated temperatures which make them suitable candidates for pharmaceutical, cosmetics and food industries. This study aims to identify potential of a novel thermophile for the production of high amounts of exopolysaccharide. It also reports suitable process parameters for growth of novel thermophile in exopolysaccharide production medium and determination of optimum glucose concentration as its production generally requires high amount of glucose making the process cost intensive. Thermophilic bacteria, from a local hot spring, were screened for the production of exopolysaccharide, and growth kinetics of the best producer, Brevibacillus borstelensis MK878423, was studied. The growth study of bacterium revealed optimum time of production as 40 h, and maximum product was formed at 30 g/l glucose. The logistic growth curve of the bacterium was analysed, and kinetic rate constants for substrate utilization were estimated by modified Luedeking-Piret kinetic model. The specific growth rate of the bacterium was calculated to be 0.0166 $ h^{−1} $ with kinetic rate constants as − 18.953 (α) and 90.655 (β) for Luedeking-Piret substrate. This is the first report on the production of exopolysaccharide by a strain of Brevibacillus borstelensis. Understanding the kinetic rate constants for substrate consumption will help in extrapolating the system of microbial exopolysaccharide production from agricultural wastes with high glucose content. The determination of optimal glucose concentration will also help in utilizing biowastes as substrates for exopolysaccharide production following biorefinery concept as wastes are utilized for the production of value-added chemicals..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Biomass Conversion and Biorefinery - 13(2021), 9 vom: 04. Feb., Seite 7521-7531 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dhagat, Swasti [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Exopolysaccharide |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021 |
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| doi: |
10.1007/s13399-021-01288-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Microbial exopolysaccharides, especially from thermophiles, are gaining attention due to their thermostability and reduced viscosity at elevated temperatures which make them suitable candidates for pharmaceutical, cosmetics and food industries. This study aims to identify potential of a novel thermophile for the production of high amounts of exopolysaccharide. It also reports suitable process parameters for growth of novel thermophile in exopolysaccharide production medium and determination of optimum glucose concentration as its production generally requires high amount of glucose making the process cost intensive. Thermophilic bacteria, from a local hot spring, were screened for the production of exopolysaccharide, and growth kinetics of the best producer, Brevibacillus borstelensis MK878423, was studied. The growth study of bacterium revealed optimum time of production as 40 h, and maximum product was formed at 30 g/l glucose. The logistic growth curve of the bacterium was analysed, and kinetic rate constants for substrate utilization were estimated by modified Luedeking-Piret kinetic model. The specific growth rate of the bacterium was calculated to be 0.0166 $ h^{−1} $ with kinetic rate constants as − 18.953 (α) and 90.655 (β) for Luedeking-Piret substrate. This is the first report on the production of exopolysaccharide by a strain of Brevibacillus borstelensis. Understanding the kinetic rate constants for substrate consumption will help in extrapolating the system of microbial exopolysaccharide production from agricultural wastes with high glucose content. The determination of optimal glucose concentration will also help in utilizing biowastes as substrates for exopolysaccharide production following biorefinery concept as wastes are utilized for the production of value-added chemicals. | ||
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| 700 | 1 | |a Jujjavarapu, Satya Eswari |0 (orcid)0000-0002-1754-961X |4 aut | |
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