Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats
Abstract Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, $ O_{3} $, and As + $ O_{3.} $ As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) $ O_{3} $ in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + $ O_{3} $ and $ O_{3} $ groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue. In TUNEL assessments, similar outcomes were obtained in the control and As + $ O_{3} $ groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the $ O_{3} $ and As + $ O_{3} $ groups. There was no significant difference between the As + $ O_{3} $ and $ O_{3} $ groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + $ O_{3} $ group (p = 0.01), and the Casp-3 expression level was not significantly changed in the $ O_{3} $ group compared to the As + $ O_{3} $ group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by $ O_{3} $ administration. Thus, ozone therapy can be a treatment choice for As toxicity..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:201 |
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| Enthalten in: |
Biological trace element research - 201(2022), 8 vom: 28. Nov., Seite 3951-3960 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Büyük, Başak [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Arsenic poisoning |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s12011-022-03486-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, $ O_{3} $, and As + $ O_{3.} $ As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) $ O_{3} $ in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + $ O_{3} $ and $ O_{3} $ groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue. In TUNEL assessments, similar outcomes were obtained in the control and As + $ O_{3} $ groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the $ O_{3} $ and As + $ O_{3} $ groups. There was no significant difference between the As + $ O_{3} $ and $ O_{3} $ groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + $ O_{3} $ group (p = 0.01), and the Casp-3 expression level was not significantly changed in the $ O_{3} $ group compared to the As + $ O_{3} $ group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by $ O_{3} $ administration. Thus, ozone therapy can be a treatment choice for As toxicity. | ||
| 650 | 4 | |a Arsenic poisoning | |
| 650 | 4 | |a Ozone | |
| 650 | 4 | |a Experimental | |
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| 650 | 4 | |a Liver injury | |
| 650 | 4 | |a Renal injury | |
| 700 | 1 | |a Aydeğer, Cemre |0 (orcid)0000-0003-1654-6406 |4 aut | |
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| 700 | 1 | |a Ovalı, Mehmet Akif |0 (orcid)0000-0002-3672-871X |4 aut | |
| 700 | 1 | |a Makav, Mustafa |0 (orcid)0000-0003-1879-8180 |4 aut | |
| 700 | 1 | |a Eroğlu, Hüseyin Avni |0 (orcid)0000-0002-1040-3255 |4 aut | |
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