Details der Publikation - Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level

Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level

Abstract Echinocandin B (ECB) is the key precursor compound of the antifungal drug Anidulafungin. The effects of the five precursor amino acids on ECB biosynthesis were firstly investigated. It showed that although L-threonine was a main compound of the hexapeptide scaffold of ECB, exogenous addition of L-threonine had no significant effect on the increase of ECB fermentation titer. Meanwhile, the ECB fermentation titer with methyl oleate showed two times higher than that of the other carbon sources. Transcription level analysis of the key genes for ECB biosynthesis indicated that the gene an655543 related to L-threonine biosynthesis showed higher value during the fermentation process, therefore, the exogenous addition of L-threonine had no obvious affection. Furthermore, it indicated that the transcription level of gene ecdA might be the main restriction factor for the ECB biosynthesis. The study provided the research foundation for the modification of the ECB producing strains in the following work..

Medienart:	E-Artikel

Erscheinungsjahr:	2023
Erschienen:	2023

Enthalten in:	Zur Gesamtaufnahme - volume:46
Enthalten in:	Bioprocess and biosystems engineering - 46(2023), 7 vom: 30. Mai, Seite 1045-1052

Sprache:	Englisch

Beteiligte Personen:	Niu, Kun [VerfasserIn] Qi, Yu-Xin [VerfasserIn] Cai, Hong-Wei [VerfasserIn] Ye, Yi-Xin [VerfasserIn] Zhou, Hai-Yan [VerfasserIn] Liu, Xiao-Tian [VerfasserIn] Liu, Zhi-Qiang [VerfasserIn] Zheng, Yu-Guo [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	58.30$jBiotechnologie 58.00$jChemische Technik: Allgemeines
Themen:	Echinocandin B Methyl oleate Precursor amino acids Transcription level analysis

RVK:	RVK Klassifikation ELIB10 ELIB09 ELIB24 ELIB41

Anmerkungen:	© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:	10.1007/s00449-023-02883-4

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2143676670

Internformat


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520			\|a Abstract Echinocandin B (ECB) is the key precursor compound of the antifungal drug Anidulafungin. The effects of the five precursor amino acids on ECB biosynthesis were firstly investigated. It showed that although L-threonine was a main compound of the hexapeptide scaffold of ECB, exogenous addition of L-threonine had no significant effect on the increase of ECB fermentation titer. Meanwhile, the ECB fermentation titer with methyl oleate showed two times higher than that of the other carbon sources. Transcription level analysis of the key genes for ECB biosynthesis indicated that the gene an655543 related to L-threonine biosynthesis showed higher value during the fermentation process, therefore, the exogenous addition of L-threonine had no obvious affection. Furthermore, it indicated that the transcription level of gene ecdA might be the main restriction factor for the ECB biosynthesis. The study provided the research foundation for the modification of the ECB producing strains in the following work.
650		4	\|a Echinocandin B
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700	1		\|a Zheng, Yu-Guo \|4 aut
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Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level

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