Details der Publikation - Mitochondrial DNA mutation affects the pluripotency of embryonic stem cells with metabolism modulation

Mitochondrial DNA mutation affects the pluripotency of embryonic stem cells with metabolism modulation

Abstract Mitochondria are the only organelles other than the nucleus harboring their DNA in mammalian cells. The mitochondrial DNA (mtDNA) mutation is the cause of many serious diseases, which still lack effective therapies. Our previous report showed that mtDNA mutation exacerbates female reproductive aging. However, the regulation of mtDNA mutation on stem cells is still unknown. Herein, we generated embryonic stem cells (ESCs) harboring massive mtDNA mutations without affecting genomic DNA to study the role of mtDNA mutation in pluripotency. The mtDNA mutation impacted the balance of pluripotency and totipotency of ESCs with a metabolism modulation as the down-regulation of oxidative phosphorylation in energy production, followed by a high level of ROS production by mitochondria. This work would shed light on the investigation and treatment of mtDNA mutant diseases..

Medienart:	E-Artikel

Erscheinungsjahr:	2022
Erschienen:	2022

Enthalten in:	Zur Gesamtaufnahme - volume:4
Enthalten in:	Genome instability & disease - 4(2022), 1 vom: 05. Dez., Seite 12-20

Sprache:	Englisch

Beteiligte Personen:	Qi, Juntao [VerfasserIn] Long, Qi [VerfasserIn] Yuan, Yang [VerfasserIn] Zhou, Yanshuang [VerfasserIn] Zhang, Jian [VerfasserIn] Ruan, Zifeng [VerfasserIn] Yang, Liang [VerfasserIn] Wu, Yi [VerfasserIn] Xiang, Ge [VerfasserIn] Li, Wei [VerfasserIn] Wu, Hao [VerfasserIn] Du, Shiwei [VerfasserIn] Liu, Xingguo [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	ESC Mitochondria MtDNA MtDNA mutation Stem cell

Anmerkungen:	© Shenzhen University School of Medicine; Fondazione Istituto FIRC di Oncologia Molecolare 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:	10.1007/s42764-022-00093-y

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2134023163

Internformat


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520			\|a Abstract Mitochondria are the only organelles other than the nucleus harboring their DNA in mammalian cells. The mitochondrial DNA (mtDNA) mutation is the cause of many serious diseases, which still lack effective therapies. Our previous report showed that mtDNA mutation exacerbates female reproductive aging. However, the regulation of mtDNA mutation on stem cells is still unknown. Herein, we generated embryonic stem cells (ESCs) harboring massive mtDNA mutations without affecting genomic DNA to study the role of mtDNA mutation in pluripotency. The mtDNA mutation impacted the balance of pluripotency and totipotency of ESCs with a metabolism modulation as the down-regulation of oxidative phosphorylation in energy production, followed by a high level of ROS production by mitochondria. This work would shed light on the investigation and treatment of mtDNA mutant diseases.
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Mitochondrial DNA mutation affects the pluripotency of embryonic stem cells with metabolism modulation

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