Thymoquinone counteracts oxidative and inflammatory machinery in carrageenan-induced murine paw edema model

Abstract Thymoquinone (TQ) is an active constituent in Nigella sativa (black cumin) and is extensively reported for its distinguished antioxidant and anti-inflammatory bioactivities. Despite the local protective response of acute inflammation, it contributes to the development of various disease conditions such as cell death, organ damage, or carcinogenesis. Hence, in this study, the effects of orally administered TQ (50 mg/kg and 100 mg/kg) for 14 days against edema development, oxidative stress, and inflammation were investigated in paw edema induced by carrageenan in mice. Indomethacin (10 mg/kg) was used as a reference drug. The results revealed that TQ reduced the paw edema volume in a time-dependent manner, attenuated acetic acid-provoked writhing movements, and reduced xylene-triggered ear edema. Hematological findings revealed marked normalization of altered counts of WBCs, and platelets. Furthermore, paw tissue levels of malondialdehyde and nitric oxide showed marked decreases together with increases in nuclear factor erythroid 2-related factor 2, glutathione, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase after TQ administration. Additionally, TQ decreased pro-inflammatory mediators, such as interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6, monocyte chemoattractant protein-1, C-reactive protein, myeloperoxidase, and nuclear factor kappa-B in the inflamed paw tissue. Moreover, appreciable decreases were recorded in cyclooxygenase-2 and its product prostaglandin E2 and the immune reaction of tumor necrosis factor-alpha in TQ-treated mice. Histopathological findings further validated the potential antiedematous, anti-inflammatory power of TQ in inflamed tissues. Conclusively, the results encourage the potent application of TQ to subside acute inflammatory events because of its striking antioxidant and anti-inflammatory properties in inflamed paw tissue..

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:30

Enthalten in:

Environmental science and pollution research - 30(2022), 6 vom: 03. Okt., Seite 16597-16611

Sprache:

Englisch

Beteiligte Personen:

Hijazy, Hayfa Hussin Ali [VerfasserIn]
Dahran, Naief [VerfasserIn]
Althagafi, Hussam A. [VerfasserIn]
Alharthi, Fahad [VerfasserIn]
Habotta, Ola A. [VerfasserIn]
Oyouni, Atif Abdulwahab A. [VerfasserIn]
Algahtani, Mohammad [VerfasserIn]
Theyab, Abdulrahman [VerfasserIn]
Al-Amer, Osama [VerfasserIn]
Lokman, Maha S. [VerfasserIn]
Alsharif, Khalaf F. [VerfasserIn]
Albrakati, Ashraf [VerfasserIn]
Amin, Hatem K. [VerfasserIn]
Dawood, Shauq Mumtaz [VerfasserIn]
Kassab, Rami B. [VerfasserIn]
Ellethy, Rania A. [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

Themen:

Histopathology
Inflammatory mediators
Paw edema
Redox status
Thymoquinone

Anmerkungen:

© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:

10.1007/s11356-022-23343-8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC213383933X

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