Colorimetric platform based on synergistic effect between bacteriophage and AuPt nanozyme for determination of Yersinia pseudotuberculosis
The development of a novel colorimetric method is reported, using vB_YepM_ZN18 phages along with AuPt nanozyme for the sensitive detection of Y. pseudotuberculosis. The phage used in this work has been extracted from hospital sewer water and is highly specific toward Y. pseudotuberculosis. The synthesized AuPt NPs possess peroxidase-like activity, which is suitable in the development of nanozyme based detection system. Furthermore, phagesMB and AuPt@phages are added into the bacterial samples for co-incubation, forming an intercalated complex. The magnetic separation and absorbance analysis of enzymatic reaction are carried out for the detection of targeted bacteria. The proposed method has a limit of detection of 14 CFU/mL, a wide linear range from 2.50 × $ 10^{1} $ ~ 2.50 × $ 10^{7} $ CFU/mL and the assay completion time is 40 min. Benefitting from the outperformance of this sensor, we have successfully employed the developed sensing platform for the detection of Y. pseudotuberculosis in food industry and hospital specimens. Graphical Abstract.
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Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:190 |
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| Enthalten in: |
Microchimica acta ... - 190(2023), 2 vom: 28. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Qiaoli [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
BSA blocking |
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© The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s00604-023-05643-z |
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| funding: |
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| 520 | |a The development of a novel colorimetric method is reported, using vB_YepM_ZN18 phages along with AuPt nanozyme for the sensitive detection of Y. pseudotuberculosis. The phage used in this work has been extracted from hospital sewer water and is highly specific toward Y. pseudotuberculosis. The synthesized AuPt NPs possess peroxidase-like activity, which is suitable in the development of nanozyme based detection system. Furthermore, phagesMB and AuPt@phages are added into the bacterial samples for co-incubation, forming an intercalated complex. The magnetic separation and absorbance analysis of enzymatic reaction are carried out for the detection of targeted bacteria. The proposed method has a limit of detection of 14 CFU/mL, a wide linear range from 2.50 × $ 10^{1} $ ~ 2.50 × $ 10^{7} $ CFU/mL and the assay completion time is 40 min. Benefitting from the outperformance of this sensor, we have successfully employed the developed sensing platform for the detection of Y. pseudotuberculosis in food industry and hospital specimens. Graphical Abstract | ||
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| 700 | 1 | |a Wu, Dan |4 aut | |
| 700 | 1 | |a Aziz, Ayesha |4 aut | |
| 700 | 1 | |a Deng, Sangsang |4 aut | |
| 700 | 1 | |a Zhou, Lei |4 aut | |
| 700 | 1 | |a Chen, Wei |4 aut | |
| 700 | 1 | |a Asif, Muhammad |4 aut | |
| 700 | 1 | |a Wang, Shenqi |4 aut | |
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