Metabolic and tissue-specific expression profiling in micropropagated plants of Malaxis acuminata: an endangered medicinal orchid

Abstract Malaxis acuminata D. Don [= Crepidium acuminatum (D. Don) Szlach.] is an important medicinal orchid used in various pharmaceutical preparations including “Ayurveda”. We report an improvised regeneration protocol for this prized orchid species using transverse thin cell layer (t-TCL) explants. The highest rate of shoot proliferation was recorded in the medium supplemented with meta-Topolin (mT) and chitosan at concentrations of 1.5 mg/l and 5.0 mg/l, respectively, wherein an average of 21 micro-shoots/explant were produced. The micro-shoots were transferred to rooting medium containing auxin [indole-3-butyric acid (IBA)/indole-3-acetic acid (IAA)] and phenolic elicitor [phloroglucinol (PG)]. The best rooting frequency was observed using 1.5 mg/l IBA and 5.0 mg/l PG in the culture medium. The regenerated plants with proliferated roots and shoots were acclimatized and survival rate was recorded. To evaluate the phytomedicinal efficiency of the micropropagated plants, flavonoid and phenolic compositions were determined using ultra performance liquid chromatography (UPLC). Four prominent fractions of metabolites were detected, of which the highest contents of quercetin and syringic acid were found in pseudobulbs and protocorm-like bodies (PLBs). Also, the highest antioxidant activities were recorded in the pseudobulb followed by PLB, leaf and whole plant extracts. The principal component analysis depicted the association between the plant organs/tissues and their metabolite contents. Furthermore, the expression levels of the five key modulator genes [viz. chalcone synthase (chs); phenylalanine ammonia lyase (pal); cinnamoyl-CoA reductase (ccr); hydroxymethylglutaryl-CoA-reductase (hmgr) and diphosphomevalonate decarboxylase (mvdd)] belonging to the flavonoid and phenolic acid biosynthetic pathways were studied in order to map the transcript abundance in various tissue parts of M. acuminata through quantitative real-time polymerase chain reaction (RT-qPCR). The present protocol can serve as a model approach for sustainable utilization of endangered orchid species of high industrial demand, strengthening the utility of chitosan as growth promoter in orchid micropropagation..

Key message The research reports the use of meta-Topolin (mT)- chitosan synergy depicting gene-metabolite relation with the scope of adapting it as a model approach for bioprospection and conservation of orchids..

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:151

Enthalten in:

Plant cell, tissue and organ culture - 151(2022), 3 vom: 07. Okt., Seite 535-549

Sprache:

Englisch

Beteiligte Personen:

Bhattacharyya, Paromik [VerfasserIn]
Lalthafamkimi, Lucy [VerfasserIn]
Sharma, Tanvi [VerfasserIn]
Kumar, Dinesh [VerfasserIn]
Kumar, Anil [VerfasserIn]
Kumar, Dinesh [VerfasserIn]
Kumar, Sanjay [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

BKL:

42.03$jMethoden und Techniken der Biologie

42.40$jPflanzencytologie$jPflanzenhistologie$jPflanzenmorphologie

48.03$jMethoden und Techniken der Land- und Forstwirtschaft

48.56$jPflanzenvermehrung

Themen:

-Topolin
Biostimulants
Orchids
Organic elicitors
Phytomedicine

Anmerkungen:

© The Author(s), under exclusive licence to Springer Nature B.V. 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

doi:

10.1007/s11240-022-02369-3

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2132923140

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