Genome-Wide Identification, Gene Structure, and Expression Analyses of the NtPP2C Gene Family in Nicotiana tabacum in Response to Low Temperature, Salt, and Drought Conditions
Abstract In plants, members of the large PP2C gene family regulate development, growth, and responses to stress exposure. While the tobacco genome is now readily available, there have been few analyses regarding the evolution, expression, and function of PP2C family genes in tobacco plants. Herein, a genome-wide identification strategy was used to explore PP2C gene structures, motifs, and expression patterns in different Nicotiana tabacum L. tissues, with their expression further being evaluated via real-time PCR under a range of stress conditions. In total, 183 NtPP2C genes were identified within the tobacco genome, and phylogenetic analyses revealed these genes to harbor similar gene structures and conserved motifs, and to be clustered within the same clade. The tissue-specific expression patterns of NtPP2C genes from Clade A were additionally characterized. Notably, NtPP2C11 and NtPP2C12 exhibited the highest levels of expression, while NtPP2C18 and NtPP2C19 transcripts were largely undetectable. Specific NtPP2C genes were found to be upregulated in response to low temperature, salt, and drought treatment conditions. Of these, NtPP2C11 and NtPP2C12 were significantly upregulated in response to these treatments, suggesting that they may play important roles in regulating plant responses to exogenous stressors. Overall, these data provide a robust foundation for further functional analyses of NtPP2C genes in tobacco..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:69 |
|---|---|
| Enthalten in: |
Russian journal of plant physiology - 69(2022), 5 vom: 20. Sept. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Gao, Y. L. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: |
|---|
| Anmerkungen: |
© Pleiades Publishing, Ltd. 2022. ISSN 1021-4437, Russian Journal of Plant Physiology, 2022, Vol. 69:91. © Pleiades Publishing, Ltd., 2022. |
|---|
| doi: |
10.1134/S1021443722050065 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2132099995 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2132099995 | ||
| 003 | DE-627 | ||
| 005 | 20230506151314.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230506s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1134/S1021443722050065 |2 doi | |
| 035 | |a (DE-627)OLC2132099995 | ||
| 035 | |a (DE-He213)S1021443722050065-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 580 |q VZ |
| 084 | |a 42.00$jBiologie: Allgemeines |2 bkl | ||
| 100 | 1 | |a Gao, Y. L. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genome-Wide Identification, Gene Structure, and Expression Analyses of the NtPP2C Gene Family in Nicotiana tabacum in Response to Low Temperature, Salt, and Drought Conditions |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Pleiades Publishing, Ltd. 2022. ISSN 1021-4437, Russian Journal of Plant Physiology, 2022, Vol. 69:91. © Pleiades Publishing, Ltd., 2022. | ||
| 520 | |a Abstract In plants, members of the large PP2C gene family regulate development, growth, and responses to stress exposure. While the tobacco genome is now readily available, there have been few analyses regarding the evolution, expression, and function of PP2C family genes in tobacco plants. Herein, a genome-wide identification strategy was used to explore PP2C gene structures, motifs, and expression patterns in different Nicotiana tabacum L. tissues, with their expression further being evaluated via real-time PCR under a range of stress conditions. In total, 183 NtPP2C genes were identified within the tobacco genome, and phylogenetic analyses revealed these genes to harbor similar gene structures and conserved motifs, and to be clustered within the same clade. The tissue-specific expression patterns of NtPP2C genes from Clade A were additionally characterized. Notably, NtPP2C11 and NtPP2C12 exhibited the highest levels of expression, while NtPP2C18 and NtPP2C19 transcripts were largely undetectable. Specific NtPP2C genes were found to be upregulated in response to low temperature, salt, and drought treatment conditions. Of these, NtPP2C11 and NtPP2C12 were significantly upregulated in response to these treatments, suggesting that they may play important roles in regulating plant responses to exogenous stressors. Overall, these data provide a robust foundation for further functional analyses of NtPP2C genes in tobacco. | ||
| 700 | 1 | |a Chen, X. J. |4 aut | |
| 700 | 1 | |a Jiao, F. C. |4 aut | |
| 700 | 1 | |a Wu, X. F. |4 aut | |
| 700 | 1 | |a Li, Y. P. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Russian journal of plant physiology |d Pleiades Publishing, 1996 |g 69(2022), 5 vom: 20. Sept. |h Online-Ressource |w (DE-627)324825692 |w (DE-600)2031151-5 |w (DE-576)121192164 |x 1608-3407 |7 nnns |
| 773 | 1 | 8 | |g volume:69 |g year:2022 |g number:5 |g day:20 |g month:09 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1134/S1021443722050065 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a GBV_ILN_11 | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_152 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_206 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2474 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
| 936 | b | k | |a 42.00$jBiologie: Allgemeines |q VZ |0 106419943 |0 (DE-625)106419943 |
| 951 | |a AR | ||
| 952 | |d 69 |j 2022 |e 5 |b 20 |c 09 | ||