Degradation of 1,4-dioxane by Newly Isolated Acinetobacter sp. M21 with Molasses as the Auxiliary Substrate
Abstract The elimination of 1,4-dioxane (dioxane), a persistent organic pollutant, is a great challenge owing to its high hydrophilicity and chemical stability. Cometabolic bioremediation technology is an effective approach to remove many organic pollutants. Because of its eco-friendly and inexpensive properties, molasses is widely used as an auxiliary biomaterial to clean up compound-contaminated sites. In this study, a newly isolated bacterium Acinetobacter sp. M21 could effectively remove dioxane using molasses without any apparent lag phase. Under the optimized molasses dosage of 0.3%, M21 could remove 500 mg/L dioxane by 60.0 ± 2.8% within 20 days with a maximum dioxane degradation rate of 1.3 ± 0.2 mg-dioxane/L/h in the first day, and exhibited extraordinary dioxane tolerance up to 1,000 mg/L, while so high dose of dioxane negatively affected the cell growth. The degradation pathway of dioxane was also determined, and was supported by the detection of 2-hydroxyethoxyacetic acid as the key metabolite of dioxane. High level degradation activity of M21 to 20 mg/L dioxane was maintained over a variable of pH (5–11), temperatures (15–45°C), and salinities (up to 8%, as NaCl wt). This is the first report linking the cometabolism of dioxane and molasses by Acinetobacter sp. M21, a bacterium that shows great potential for field dioxane bioremediation..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
Biotechnology and bioprocess engineering - 27(2022), 3 vom: Juni, Seite 423-431 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Peng [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
1,4-dioxane |
| Anmerkungen: |
© The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
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| doi: |
10.1007/s12257-021-0212-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2131205783 |
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| 520 | |a Abstract The elimination of 1,4-dioxane (dioxane), a persistent organic pollutant, is a great challenge owing to its high hydrophilicity and chemical stability. Cometabolic bioremediation technology is an effective approach to remove many organic pollutants. Because of its eco-friendly and inexpensive properties, molasses is widely used as an auxiliary biomaterial to clean up compound-contaminated sites. In this study, a newly isolated bacterium Acinetobacter sp. M21 could effectively remove dioxane using molasses without any apparent lag phase. Under the optimized molasses dosage of 0.3%, M21 could remove 500 mg/L dioxane by 60.0 ± 2.8% within 20 days with a maximum dioxane degradation rate of 1.3 ± 0.2 mg-dioxane/L/h in the first day, and exhibited extraordinary dioxane tolerance up to 1,000 mg/L, while so high dose of dioxane negatively affected the cell growth. The degradation pathway of dioxane was also determined, and was supported by the detection of 2-hydroxyethoxyacetic acid as the key metabolite of dioxane. High level degradation activity of M21 to 20 mg/L dioxane was maintained over a variable of pH (5–11), temperatures (15–45°C), and salinities (up to 8%, as NaCl wt). This is the first report linking the cometabolism of dioxane and molasses by Acinetobacter sp. M21, a bacterium that shows great potential for field dioxane bioremediation. | ||
| 650 | 4 | |a 1,4-dioxane | |
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| 650 | 4 | |a cometabolic biodegradation | |
| 650 | 4 | |a biotransformation | |
| 700 | 1 | |a Cui, Tingchen |4 aut | |
| 700 | 1 | |a Yang, Yadong |4 aut | |
| 700 | 1 | |a Li, Jialu |4 aut | |
| 700 | 1 | |a Su, Yaoming |4 aut | |
| 700 | 1 | |a Liu, Na |4 aut | |
| 700 | 1 | |a Hong, Mei |4 aut | |
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