Details der Publikation - Nandrolone Supplementation Promotes AMPK Activation and Divergent 18[FDG] PET Brain Connectivity in Adult and Aged Mice

Nandrolone Supplementation Promotes AMPK Activation and Divergent 18[FDG] PET Brain Connectivity in Adult and Aged Mice

Abstract Decreased anabolic androgen levels are followed by impaired brain energy support and sensing with loss of neural connectivity during physiological aging, providing a neurobiological basis for hormone supplementation. Here, we investigated whether nandrolone decanoate (ND) administration mediates hypothalamic AMPK activation and glucose metabolism, thus affecting metabolic connectivity in brain areas of adult and aged mice. Metabolic interconnected brain areas of rodents can be detected by positron emission tomography using 18FDG-mPET. Albino CF1 mice at 3 and 18 months of age were separated into 4 groups that received daily subcutaneous injections of either ND (15 mg/kg) or vehicle for 15 days. At the in vivo baseline and on the 14th day, brain 18FDG-microPET scans were performed. Hypothalamic $ pAMPK^{T172} $/AMPK protein levels were assessed, and basal mitochondrial respiratory states were evaluated in synaptosomes. A metabolic connectivity network between brain areas was estimated based on 18FDG uptake. We found that ND increased the $ pAMPK^{T172} $/AMPK ratio in both adult and aged mice but increased 18FDG uptake and mitochondrial basal respiration only in adult mice. Furthermore, ND triggered rearrangement in the metabolic connectivity of adult mice and aged mice compared to age-matched controls. Altogether, our findings suggest that ND promotes hypothalamic AMPK activation, and distinct glucose metabolism and metabolic connectivity rearrangements in the brains of adult and aged mice..

Medienart:	E-Artikel

Erscheinungsjahr:	2022
Erschienen:	2022

Enthalten in:	Zur Gesamtaufnahme - volume:47
Enthalten in:	Neurochemical research - 47(2022), 7 vom: 12. Apr., Seite 2032-2042

Sprache:	Englisch

Beteiligte Personen:	Strogulski, N. R. [VerfasserIn] Kopczynski, A. [VerfasserIn] de Oliveira, V. G. [VerfasserIn] Carteri, R. B. [VerfasserIn] Hansel, G. [VerfasserIn] Venturin, G. T. [VerfasserIn] Greggio, S. [VerfasserIn] DaCosta, J. C. [VerfasserIn] De Bastiani, M. A. [VerfasserIn] Rodolphi, M. S. [VerfasserIn] Portela, L. V. [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	44.90$jNeurologie
Themen:	Aging Anabolic-androgen steroids FDG-PET Mitochondria Nandrolone decanoate Neural metabolic connectivity

Anmerkungen:	© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022

doi:	10.1007/s11064-022-03592-2

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2131026382

Internformat


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520			\|a Abstract Decreased anabolic androgen levels are followed by impaired brain energy support and sensing with loss of neural connectivity during physiological aging, providing a neurobiological basis for hormone supplementation. Here, we investigated whether nandrolone decanoate (ND) administration mediates hypothalamic AMPK activation and glucose metabolism, thus affecting metabolic connectivity in brain areas of adult and aged mice. Metabolic interconnected brain areas of rodents can be detected by positron emission tomography using 18FDG-mPET. Albino CF1 mice at 3 and 18 months of age were separated into 4 groups that received daily subcutaneous injections of either ND (15 mg/kg) or vehicle for 15 days. At the in vivo baseline and on the 14th day, brain 18FDG-microPET scans were performed. Hypothalamic $ pAMPK^{T172} $/AMPK protein levels were assessed, and basal mitochondrial respiratory states were evaluated in synaptosomes. A metabolic connectivity network between brain areas was estimated based on 18FDG uptake. We found that ND increased the $ pAMPK^{T172} $/AMPK ratio in both adult and aged mice but increased 18FDG uptake and mitochondrial basal respiration only in adult mice. Furthermore, ND triggered rearrangement in the metabolic connectivity of adult mice and aged mice compared to age-matched controls. Altogether, our findings suggest that ND promotes hypothalamic AMPK activation, and distinct glucose metabolism and metabolic connectivity rearrangements in the brains of adult and aged mice.
650		4	\|a Aging
650		4	\|a Mitochondria
650		4	\|a FDG-PET
650		4	\|a Anabolic-androgen steroids
650		4	\|a Nandrolone decanoate
650		4	\|a Neural metabolic connectivity
700	1		\|a Kopczynski, A. \|4 aut
700	1		\|a de Oliveira, V. G. \|4 aut
700	1		\|a Carteri, R. B. \|4 aut
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700	1		\|a Venturin, G. T. \|4 aut
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700	1		\|a DaCosta, J. C. \|4 aut
700	1		\|a De Bastiani, M. A. \|4 aut
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Nandrolone Supplementation Promotes AMPK Activation and Divergent 18[FDG] PET Brain Connectivity in Adult and Aged Mice

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