CD19 + $ CD21^{lo/neg} $ cells are increased in systemic sclerosis-associated interstitial lung disease

Abstract Interstitial lung disease (ILD) represents a significant cause of morbidity and mortality in systemic sclerosis (SSc). The purpose of this study was to examine recirculating lymphocytes from SSc patients for potential biomarkers of interstitial lung disease (ILD). Peripheral blood mononuclear cells (PBMCs) were isolated from patients with SSc and healthy controls enrolled in the Vanderbilt University Myositis and Scleroderma Treatment Initiative Center cohort between 9/2017–6/2019. Clinical phenotyping was performed by chart abstraction. Immunophenotyping was performed using both mass cytometry and fluorescence cytometry combined with t-distributed stochastic neighbor embedding analysis and traditional biaxial gating. This study included 34 patients with SSc-ILD, 14 patients without SSc-ILD, and 25 healthy controls. $ CD21^{lo/neg} $ cells are significantly increased in SSc-ILD but not in SSc without ILD (15.4 ± 13.3% vs. 5.8 ± 0.9%, p = 0.002) or healthy controls (5.0 ± 0.5%, p < 0.0001). While $ CD21^{lo/neg} $ B cells can be identified from a single biaxial gate, tSNE analysis reveals that the biaxial gate is comprised of multiple distinct subsets, all of which are increased in SSc-ILD. $ CD21^{lo/neg} $ cells in both healthy controls and SSc-ILD are predominantly tBET positive and do not have intracellular CD21. Immunohistochemistry staining demonstrated that $ CD21^{lo/neg} $ B cells diffusely infiltrate the lung parenchyma of an SSc-ILD patient. Additional work is needed to validate this biomarker in larger cohorts and longitudinal studies and to understand the role of these cells in SSc-ILD..

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:22

Enthalten in:

Clinical and experimental medicine - 22(2021), 2 vom: 10. Aug., Seite 209-220

Sprache:

Englisch

Beteiligte Personen:

Wilfong, Erin M. [VerfasserIn]
Vowell, Katherine N. [VerfasserIn]
Bunn, Kaitlyn E. [VerfasserIn]
Rizzi, Elise [VerfasserIn]
Annapureddy, Narender [VerfasserIn]
Dudenhofer, Rosemarie B. [VerfasserIn]
Barnado, April [VerfasserIn]
Bonami, Rachel H. [VerfasserIn]
Johnson, Joyce E. [VerfasserIn]
Crofford, Leslie J. [VerfasserIn]
Kendall, Peggy L. [VerfasserIn]

Links:

Volltext [kostenfrei]

BKL:

44.60$jMedizinische Spezialfächer: Allgemeines

Themen:

CD21lo B cells
Interstitial lung disease
Mass cytometry
Systemic sclerosis

Anmerkungen:

© The Author(s) 2021

doi:

10.1007/s10238-021-00745-5

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2130523234

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