Details der Publikation - Elevated Atmospheric $ CO_{2} $ Modifies Mostly the Metabolic Active Rhizosphere Soil Microbiome in the Giessen FACE Experiment

Elevated Atmospheric $ CO_{2} $ Modifies Mostly the Metabolic Active Rhizosphere Soil Microbiome in the Giessen FACE Experiment

Abstract Elevated levels of atmospheric $ CO_{2} $ lead to the increase of plant photosynthetic rates, carbon inputs into soil and root exudation. In this work, the effects of rising atmospheric $ CO_{2} $ levels on the metabolic active soil microbiome have been investigated at the Giessen free-air $ CO_{2} $ enrichment (Gi-FACE) experiment on a permanent grassland site near Giessen, Germany. The aim was to assess the effects of increased C supply into the soil, due to elevated $ CO_{2} $, on the active soil microbiome composition. RNA extraction and 16S rRNA (cDNA) metabarcoding sequencing were performed from bulk and rhizosphere soils, and the obtained data were processed for a compositional data analysis calculating diversity indices and differential abundance analyses. The structure of the metabolic active microbiome in the rhizospheric soil showed a clear separation between elevated and ambient $ CO_{2} $ (p = 0.002); increased atmospheric $ CO_{2} $ concentration exerted a significant influence on the microbiomes differentiation (p = 0.01). In contrast, elevated $ CO_{2} $ had no major influence on the structure of the bulk soil microbiome (p = 0.097). Differential abundance results demonstrated that 42 bacterial genera were stimulated under elevated $ CO_{2} $. The RNA-based metabarcoding approach used in this research showed that the ongoing atmospheric $ CO_{2} $ increase of climate change will significantly shift the microbiome structure in the rhizosphere..

Medienart:	E-Artikel

Erscheinungsjahr:	2021
Erschienen:	2021

Enthalten in:	Zur Gesamtaufnahme - volume:83
Enthalten in:	Microbial ecology - 83(2021), 3 vom: 19. Juni, Seite 619-634

Sprache:	Englisch

Beteiligte Personen:	Rosado-Porto, David [VerfasserIn] Ratering, Stefan [VerfasserIn] Cardinale, Massimiliano [VerfasserIn] Maisinger, Corinna [VerfasserIn] Moser, Gerald [VerfasserIn] Deppe, Marianna [VerfasserIn] Müller, Christoph [VerfasserIn] Schnell, Sylvia [VerfasserIn]

Links:	Volltext [kostenfrei]

BKL:	42.90$jÖkologie: Allgemeines
Themen:	Elevated CO Grassland RNA metabarcoding Rhizosphere microbiome

RVK:	RVK Klassifikation ELIB09

Anmerkungen:	© The Author(s) 2021

doi:	10.1007/s00248-021-01791-y

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2130025463

Internformat


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520			\|a Abstract Elevated levels of atmospheric $ CO_{2} $ lead to the increase of plant photosynthetic rates, carbon inputs into soil and root exudation. In this work, the effects of rising atmospheric $ CO_{2} $ levels on the metabolic active soil microbiome have been investigated at the Giessen free-air $ CO_{2} $ enrichment (Gi-FACE) experiment on a permanent grassland site near Giessen, Germany. The aim was to assess the effects of increased C supply into the soil, due to elevated $ CO_{2} $, on the active soil microbiome composition. RNA extraction and 16S rRNA (cDNA) metabarcoding sequencing were performed from bulk and rhizosphere soils, and the obtained data were processed for a compositional data analysis calculating diversity indices and differential abundance analyses. The structure of the metabolic active microbiome in the rhizospheric soil showed a clear separation between elevated and ambient $ CO_{2} $ (p = 0.002); increased atmospheric $ CO_{2} $ concentration exerted a significant influence on the microbiomes differentiation (p = 0.01). In contrast, elevated $ CO_{2} $ had no major influence on the structure of the bulk soil microbiome (p = 0.097). Differential abundance results demonstrated that 42 bacterial genera were stimulated under elevated $ CO_{2} $. The RNA-based metabarcoding approach used in this research showed that the ongoing atmospheric $ CO_{2} $ increase of climate change will significantly shift the microbiome structure in the rhizosphere.
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Elevated Atmospheric $ CO_{2} $ Modifies Mostly the Metabolic Active Rhizosphere Soil Microbiome in the Giessen FACE Experiment

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