Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
Abstract Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:76 |
|---|---|
| Enthalten in: |
Chemické zvesti - 76(2021), 4 vom: 30. Dez., Seite 2567-2572 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
dos Santos Botelho, Deila Magna [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: | |
|---|---|
| Themen: |
High-performance liquid chromatography |
| Anmerkungen: |
© Institute of Chemistry, Slovak Academy of Sciences 2021 |
|---|
| doi: |
10.1007/s11696-021-01965-5 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC212980537X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC212980537X | ||
| 003 | DE-627 | ||
| 005 | 20230506000941.0 | ||
| 007 | tu | ||
| 008 | 230506s2021 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11696-021-01965-5 |2 doi | |
| 035 | |a (DE-627)OLC212980537X | ||
| 035 | |a (DE-He213)s11696-021-01965-5-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 540 |q VZ |
| 084 | |a 35.00 |2 bkl | ||
| 100 | 1 | |a dos Santos Botelho, Deila Magna |e verfasserin |0 (orcid)0000-0002-3047-0164 |4 aut | |
| 245 | 1 | 0 | |a Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Institute of Chemistry, Slovak Academy of Sciences 2021 | ||
| 520 | |a Abstract Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production. | ||
| 650 | 4 | |a Microscopy | |
| 650 | 4 | |a Toxin | |
| 650 | 4 | |a High-performance liquid chromatography | |
| 650 | 4 | |a Methods | |
| 700 | 1 | |a de Resende, Mário Lúcio Vilela |4 aut | |
| 700 | 1 | |a Teixeira, Alexandre Rezende |4 aut | |
| 700 | 1 | |a Santiago, Wilder Douglas |4 aut | |
| 700 | 1 | |a Pozza, Edson Ampélio |4 aut | |
| 700 | 1 | |a Moreira, Silvino Intra |4 aut | |
| 700 | 1 | |a Ramos, Juliana Barros |4 aut | |
| 700 | 1 | |a de Aquino, Sinara Oliveira |4 aut | |
| 700 | 1 | |a Ferreira, Antônio Gilberto |4 aut | |
| 700 | 1 | |a Reichel, Tharyn |4 aut | |
| 700 | 1 | |a Guerra-Guimarães, Leonor |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chemické zvesti |d Versita, 1947 |g 76(2021), 4 vom: 30. Dez., Seite 2567-2572 |w (DE-627)129938920 |w (DE-600)391411-2 |w (DE-576)015500659 |x 0037-6906 |7 nnns |
| 773 | 1 | 8 | |g volume:76 |g year:2021 |g number:4 |g day:30 |g month:12 |g pages:2567-2572 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s11696-021-01965-5 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 936 | b | k | |a 35.00 |q VZ |
| 951 | |a AR | ||
| 952 | |d 76 |j 2021 |e 4 |b 30 |c 12 |h 2567-2572 | ||