Details der Publikation - A novel LAMP assay using hot water in vacuum insulated bottle for rapid detection of the soybean red crown rot pathogen Calonectria ilicicola

A novel LAMP assay using hot water in vacuum insulated bottle for rapid detection of the soybean red crown rot pathogen Calonectria ilicicola

Abstract Red crown rot is an important disease of soybean crops worldwide. It is caused by the fungal pathogen Calonectria ilicicola. Infection by C. ilicicola results in a significant reduction in soybean yield and quality. Therefore, accurate pathogen detection in the early stages of the disease is paramount to implementing disease management promptly and minimizing losses. In this study, we developed a sensitive and specific loop-mediated isothermal amplification (LAMP) assay (TUB-Ci-LAMP) targeting the β-tubulin gene of C. ilicicola. Furthermore, an in-field TUB-Ci-LAMP assay was developed using Buffer C to perform on-site crude DNA extraction and a vacuum insulated bottle as a temperature-stable, portable container for isothermal amplification under the field environment. This assay only had positive reaction to C. ilicicola, but not to any of 22 other tested fungal or oomycete species. Under both laboratory and field conditions, genomic DNA of C. ilicicola at 0.01 ng per µL or higher was consistently detected using this assay. Additionally, the in-field assay detected C. ilicicola in artificially inoculated and naturally infected soybean plants. These findings indicate that TUB-Ci-LAMP is useful in aiding researchers, diagnosticians, and first responders on rapid and accurate detection of soybean red crown rot under both laboratory and field environments..

Medienart:	E-Artikel

Erscheinungsjahr:	2022
Erschienen:	2022

Enthalten in:	Zur Gesamtaufnahme - volume:51
Enthalten in:	Australasian plant pathology - 51(2022), 2 vom: 19. Feb., Seite 251-259

Sprache:	Englisch

Beteiligte Personen:	Lu, ChenChen [VerfasserIn] Dai, TingTing [VerfasserIn] Zhang, HaiFeng [VerfasserIn] Zeng, DanDan [VerfasserIn] Wang, YuanChao [VerfasserIn] Yang, WanFeng [VerfasserIn] Zheng, XiaoBo [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Plant disease diagnosis Soybean red crown rot

Anmerkungen:	© The Author(s) under exclusive licence to Australasian Plant Pathology Society Inc. 2022

doi:	10.1007/s13313-022-00855-y

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2129709117

Internformat


LEADER	01000naa a22002652 4500
001	OLC2129709117
003	DE-627
005	20230505235429.0
007	cr uuu---uuuuu
008	230505s2022 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1007/s13313-022-00855-y \|2 doi
035			\|a (DE-627)OLC2129709117
035			\|a (DE-He213)s13313-022-00855-y-e
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
082	0	4	\|a 630 \|a 640 \|q VZ
100	1		\|a Lu, ChenChen \|e verfasserin \|0 (orcid)0000-0002-1549-4950 \|4 aut
245	1	0	\|a A novel LAMP assay using hot water in vacuum insulated bottle for rapid detection of the soybean red crown rot pathogen Calonectria ilicicola
264		1	\|c 2022
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
500			\|a © The Author(s) under exclusive licence to Australasian Plant Pathology Society Inc. 2022
520			\|a Abstract Red crown rot is an important disease of soybean crops worldwide. It is caused by the fungal pathogen Calonectria ilicicola. Infection by C. ilicicola results in a significant reduction in soybean yield and quality. Therefore, accurate pathogen detection in the early stages of the disease is paramount to implementing disease management promptly and minimizing losses. In this study, we developed a sensitive and specific loop-mediated isothermal amplification (LAMP) assay (TUB-Ci-LAMP) targeting the β-tubulin gene of C. ilicicola. Furthermore, an in-field TUB-Ci-LAMP assay was developed using Buffer C to perform on-site crude DNA extraction and a vacuum insulated bottle as a temperature-stable, portable container for isothermal amplification under the field environment. This assay only had positive reaction to C. ilicicola, but not to any of 22 other tested fungal or oomycete species. Under both laboratory and field conditions, genomic DNA of C. ilicicola at 0.01 ng per µL or higher was consistently detected using this assay. Additionally, the in-field assay detected C. ilicicola in artificially inoculated and naturally infected soybean plants. These findings indicate that TUB-Ci-LAMP is useful in aiding researchers, diagnosticians, and first responders on rapid and accurate detection of soybean red crown rot under both laboratory and field environments.
650		4	\|a Plant disease diagnosis
650		4	\|a Soybean red crown rot
700	1		\|a Dai, TingTing \|4 aut
700	1		\|a Zhang, HaiFeng \|4 aut
700	1		\|a Zeng, DanDan \|4 aut
700	1		\|a Wang, YuanChao \|4 aut
700	1		\|a Yang, WanFeng \|4 aut
700	1		\|a Zheng, XiaoBo \|0 (orcid)0000-0002-7927-3583 \|4 aut
773	0	8	\|i Enthalten in \|t Australasian plant pathology \|d Springer Netherlands, 1972 \|g 51(2022), 2 vom: 19. Feb., Seite 251-259 \|h Online-Ressource \|w (DE-627)328185906 \|w (DE-600)2045038-2 \|w (DE-576)333679415 \|x 1448-6032 \|7 nnns
773	1	8	\|g volume:51 \|g year:2022 \|g number:2 \|g day:19 \|g month:02 \|g pages:251-259
856	4	0	\|u https://dx.doi.org/10.1007/s13313-022-00855-y \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_OLC
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2474
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 51 \|j 2022 \|e 2 \|b 19 \|c 02 \|h 251-259

A novel LAMP assay using hot water in vacuum insulated bottle for rapid detection of the soybean red crown rot pathogen Calonectria ilicicola

Zugang & Verfügbarkeit

Zugehörige Publikationen/Bände