Details der Publikation - Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation

Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation

Abstract The aim of the present study was to develop, optimize brucine-loaded transliposomes (BRC-TL) formulation for dermal delivery of brucine for skin cancer. The BRC-TL formulations were evaluated for vesicle size, entrapment efficiency, and in vitro drug release. The optimized formulation was further evaluated for skin penetration by confocal laser microscopy and dermatokinetic study. The optimized BRC-TL formulation presented sealed lamellar shaped vesicles, with vesicles size, polydispersity index, entrapment efficiency, and in vitro drug release of 136.20 ± 2.87 nm, 0.354 ± 0.02, 86.01 ± 1.27%, and 83.09 ± 2.07%, respectively. Ex vivo permeation study showed that, developed BRC-TL formulation had a 2.4-fold increment in permeation as compared to BRC suspension. Texture analysis showed that the BRC-TL gel presented firmness of 158.91 g, consistency of 615.03 g/s, cohesiveness of − 115.26 g and a viscosity index of − 472.05 g/s. The confocal images of rat skin clearly showed the deeper penetration of rhodamine B-loaded TL formulation as compared to the Rhodamine B-hydro alcoholic solution. The optimized BRC-TL formulation demonstrated significantly higher cytotoxicity than placebo liposome and BRC suspension (P < 0.05). Further, the BRC-TL nanogel treated rat skin showed a substantial increase in $ C_{Skin max} $ and $ AUC_{0–8} $ in comparison to rat skin treated with BRC conventional gel (P < 0.05). The data revealed that the developed TLs formulation could be a promising drug nanocarrier for brucine dermal delivery in the treatment of skin cancer..

Medienart:	E-Artikel

Erscheinungsjahr:	2021
Erschienen:	2021

Enthalten in:	Zur Gesamtaufnahme - volume:11
Enthalten in:	3 Biotech - 11(2021), 6 vom: 22. Mai

Sprache:	Englisch

Beteiligte Personen:	Alhakamy, Nabil A. [VerfasserIn] Aldawsari, Hibah M. [VerfasserIn] Ali, Javed [VerfasserIn] Gupta, Dipak K. [VerfasserIn] Warsi, Musarrat H. [VerfasserIn] Bilgrami, Anwar L. [VerfasserIn] Asfour, Hani Z. [VerfasserIn] Noor, Ahmad O. [VerfasserIn] Md, Shadab [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	58.30 / Biotechnologie / Biotechnologie
Themen:	Brucine Dermal Dermatokinetics Transliposomes

Anmerkungen:	© King Abdulaziz City for Science and Technology 2021

doi:	10.1007/s13205-021-02841-5

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2125647621

Internformat


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245	1	0	\|a Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation
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520			\|a Abstract The aim of the present study was to develop, optimize brucine-loaded transliposomes (BRC-TL) formulation for dermal delivery of brucine for skin cancer. The BRC-TL formulations were evaluated for vesicle size, entrapment efficiency, and in vitro drug release. The optimized formulation was further evaluated for skin penetration by confocal laser microscopy and dermatokinetic study. The optimized BRC-TL formulation presented sealed lamellar shaped vesicles, with vesicles size, polydispersity index, entrapment efficiency, and in vitro drug release of 136.20 ± 2.87 nm, 0.354 ± 0.02, 86.01 ± 1.27%, and 83.09 ± 2.07%, respectively. Ex vivo permeation study showed that, developed BRC-TL formulation had a 2.4-fold increment in permeation as compared to BRC suspension. Texture analysis showed that the BRC-TL gel presented firmness of 158.91 g, consistency of 615.03 g/s, cohesiveness of − 115.26 g and a viscosity index of − 472.05 g/s. The confocal images of rat skin clearly showed the deeper penetration of rhodamine B-loaded TL formulation as compared to the Rhodamine B-hydro alcoholic solution. The optimized BRC-TL formulation demonstrated significantly higher cytotoxicity than placebo liposome and BRC suspension (P < 0.05). Further, the BRC-TL nanogel treated rat skin showed a substantial increase in $ C_{Skin max} $ and $ AUC_{0–8} $ in comparison to rat skin treated with BRC conventional gel (P < 0.05). The data revealed that the developed TLs formulation could be a promising drug nanocarrier for brucine dermal delivery in the treatment of skin cancer.
650		4	\|a Brucine
650		4	\|a Transliposomes
650		4	\|a Dermal
650		4	\|a Dermatokinetics
700	1		\|a Aldawsari, Hibah M. \|4 aut
700	1		\|a Ali, Javed \|4 aut
700	1		\|a Gupta, Dipak K. \|4 aut
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700	1		\|a Bilgrami, Anwar L. \|4 aut
700	1		\|a Asfour, Hani Z. \|4 aut
700	1		\|a Noor, Ahmad O. \|4 aut
700	1		\|a Md, Shadab \|0 (orcid)0000-0002-9343-1066 \|4 aut
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952			\|d 11 \|j 2021 \|e 6 \|b 22 \|c 05

Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation

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