Details der Publikation - Chemical Composition of Bee Pollen and Leishmanicidal Activity of Rhusflavone

Chemical Composition of Bee Pollen and Leishmanicidal Activity of Rhusflavone

Abstract Bee pollen monofloral from Attalea funifera Mart., Arecaceae, known as piaçava, was analyzed with ultra-performance liquid chromatography coupled with diode array detector and quadrupole time-of-flight mass spectrometry for the profiling and structural characterization of the replicated compounds. The principal compound was isolated and tested against Leishmania amazonensis. Fifty-eight compounds were identified, including one sugar, ten flavonoids, two putrescine derivatives, sixteen hydroxycinnamic acid amide derivatives, two coumarate derivatives, and twenty-seven lipids. The isolated compound identified as the biflavonoid rhusflavone showed high leishmanicidal activity against both promastigote ($ IC_{50} $ = 5 μM) and amastigote (76.7% mortality at 2 μM) forms of L. amazonensis, acting via a mechanism involving reactive oxygen species production, mitochondrial dysfunction, and membrane disruption in parasites. The compound showed low toxicity to mammalian cells and human erythrocytes, with selectivity index for erythrocytes >100 and for macrophages ($ CC_{50 macrophages} $/$ IC_{50 amastigotes} $) > 10. These results indicate that rhusflavone is a promising candidate for the development of drugs against L. amazonensis..

Medienart:	E-Artikel

Erscheinungsjahr:	2021
Erschienen:	2021

Enthalten in:	Zur Gesamtaufnahme - volume:31
Enthalten in:	Revista Brasileira de farmacognosia - 31(2021), 2 vom: Apr., Seite 176-183

Sprache:	Englisch

Beteiligte Personen:	Gomes, Ayala Nara Pereira [VerfasserIn] Camara, Celso Amorim [VerfasserIn] dos Santos Sousa, Alexsandro [VerfasserIn] de Assis Ribeiro dos Santos, Francisco [VerfasserIn] de Santana Filho, Paulo Cesar [VerfasserIn] Dorneles, Gilson Pires [VerfasserIn] Romão, Pedro Roosevelt Torres [VerfasserIn] Silva, Tania Maria Sarmento [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Cytotoxicity Flavonoids Hydroxycinnamic acid amide Lipids UPLC-DAD-qTOF-MS/MS

Anmerkungen:	© Sociedade Brasileira de Farmacognosia 2021

doi:	10.1007/s43450-021-00130-z

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2125585308

Internformat


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520			\|a Abstract Bee pollen monofloral from Attalea funifera Mart., Arecaceae, known as piaçava, was analyzed with ultra-performance liquid chromatography coupled with diode array detector and quadrupole time-of-flight mass spectrometry for the profiling and structural characterization of the replicated compounds. The principal compound was isolated and tested against Leishmania amazonensis. Fifty-eight compounds were identified, including one sugar, ten flavonoids, two putrescine derivatives, sixteen hydroxycinnamic acid amide derivatives, two coumarate derivatives, and twenty-seven lipids. The isolated compound identified as the biflavonoid rhusflavone showed high leishmanicidal activity against both promastigote ($ IC_{50} $ = 5 μM) and amastigote (76.7% mortality at 2 μM) forms of L. amazonensis, acting via a mechanism involving reactive oxygen species production, mitochondrial dysfunction, and membrane disruption in parasites. The compound showed low toxicity to mammalian cells and human erythrocytes, with selectivity index for erythrocytes >100 and for macrophages ($ CC_{50 macrophages} $/$ IC_{50 amastigotes} $) > 10. These results indicate that rhusflavone is a promising candidate for the development of drugs against L. amazonensis.
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Chemical Composition of Bee Pollen and Leishmanicidal Activity of Rhusflavone

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