Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay
Abstracts The Hantaan virus (HTNV) and Seoul virus (SEOV) mutants have accumulated over time. It is important to determine whether their neutralizing epitopes have evolved, thereby making the current vaccine powerless. However, it is impossible to determine by using traditional plaque reduction neutralization test (PRNT), because it requires large numbers of live mutant strains. Pseudovirus-based neutralization assays (PBNA) were developed by employing vesicular stomatitis virus (VSV) backbone incorporated with HTNV or SEOV glycoproteins ($ VSVΔG^{*} $-HTNVG or $ VSVΔG^{*} $-SEOVG). 56 and 51 single amino acid substitutions of glycoprotein (GP) in HTNV and SEOV were selected and introduced into the reference plasmid. Then the mutant pseudoviruses were generated and tested by PBNA. The PBNA results were highly correlated with PRNT ones with R2 being 0.91 for $ VSVΔG^{*} $-HTNVG and 0.82 for $ VSVΔG^{*} $-SEOVG. 53 HTNV mutant pseudoviruses and 46 SEOV mutants were successfully generated. Importantly, by using PBNA, we found that HTNV or SEOV immunized antisera could neutralize all the corresponding 53 HTNV mutants or the 46 SEOV mutants respectively. The novel PBNA enables us to closely monitor the effectiveness of vaccines against large numbers of evolving HTNV and SEOV. And the current vaccine remains to be effective for the naturally occurring mutants..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
|---|---|
| Enthalten in: |
Virologica Sinica - 36(2020), 1 vom: 12. Juni, Seite 104-112 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ning, Tingting [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Amino acid substitution |
|---|
| Anmerkungen: |
© Wuhan Institute of Virology, CAS 2020 |
|---|
| doi: |
10.1007/s12250-020-00237-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2124453807 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2124453807 | ||
| 003 | DE-627 | ||
| 005 | 20230505090455.0 | ||
| 007 | tu | ||
| 008 | 230505s2020 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12250-020-00237-y |2 doi | |
| 035 | |a (DE-627)OLC2124453807 | ||
| 035 | |a (DE-He213)s12250-020-00237-y-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 570 |q VZ |
| 100 | 1 | |a Ning, Tingting |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Wuhan Institute of Virology, CAS 2020 | ||
| 520 | |a Abstracts The Hantaan virus (HTNV) and Seoul virus (SEOV) mutants have accumulated over time. It is important to determine whether their neutralizing epitopes have evolved, thereby making the current vaccine powerless. However, it is impossible to determine by using traditional plaque reduction neutralization test (PRNT), because it requires large numbers of live mutant strains. Pseudovirus-based neutralization assays (PBNA) were developed by employing vesicular stomatitis virus (VSV) backbone incorporated with HTNV or SEOV glycoproteins ($ VSVΔG^{*} $-HTNVG or $ VSVΔG^{*} $-SEOVG). 56 and 51 single amino acid substitutions of glycoprotein (GP) in HTNV and SEOV were selected and introduced into the reference plasmid. Then the mutant pseudoviruses were generated and tested by PBNA. The PBNA results were highly correlated with PRNT ones with R2 being 0.91 for $ VSVΔG^{*} $-HTNVG and 0.82 for $ VSVΔG^{*} $-SEOVG. 53 HTNV mutant pseudoviruses and 46 SEOV mutants were successfully generated. Importantly, by using PBNA, we found that HTNV or SEOV immunized antisera could neutralize all the corresponding 53 HTNV mutants or the 46 SEOV mutants respectively. The novel PBNA enables us to closely monitor the effectiveness of vaccines against large numbers of evolving HTNV and SEOV. And the current vaccine remains to be effective for the naturally occurring mutants. | ||
| 650 | 4 | |a Hemorrhagic fever with renal syndrome (HFRS) | |
| 650 | 4 | |a Hantaan virus (HTNV) | |
| 650 | 4 | |a Seoul virus (SEOV) | |
| 650 | 4 | |a Pseudovirus-based neutralization assay (PBNA) | |
| 650 | 4 | |a Amino acid substitution | |
| 650 | 4 | |a Vaccine | |
| 700 | 1 | |a Wang, Ling |4 aut | |
| 700 | 1 | |a Liu, Shuo |4 aut | |
| 700 | 1 | |a Ma, Jian |4 aut | |
| 700 | 1 | |a Nie, Jianhui |4 aut | |
| 700 | 1 | |a Huang, Weijin |4 aut | |
| 700 | 1 | |a Li, Xuguang |4 aut | |
| 700 | 1 | |a Li, Yuhua |0 (orcid)0000-0003-3806-8046 |4 aut | |
| 700 | 1 | |a Wang, Youchun |0 (orcid)0000-0001-9769-5141 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Virologica Sinica |d Springer Singapore, 2007 |g 36(2020), 1 vom: 12. Juni, Seite 104-112 |w (DE-627)566013746 |w (DE-600)2425649-3 |w (DE-576)9566013744 |x 1674-0769 |7 nnns |
| 773 | 1 | 8 | |g volume:36 |g year:2020 |g number:1 |g day:12 |g month:06 |g pages:104-112 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s12250-020-00237-y |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 951 | |a AR | ||
| 952 | |d 36 |j 2020 |e 1 |b 12 |c 06 |h 104-112 | ||