Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Abstract Our study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of $ AgNO_{3} $, $ FeCl_{3} $ and $ HAuCl_{4} $ solutions and incubated at ambient temperature for 3–4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50–100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene..

Highlights The present research work is focused on green synthesis of Silver iron and gold nanoparticles of isolated lycopene from tomato.Our work aimed to assess scientifically the anticancer efficacy of the developed metal nanoparticles on in vitro colon and cervical cell lines.The results of the study revealed that developed nanoparticles exhibited significant cytotoxic activity against colon cancer and cervical cancer as compared with pure lycopene (phytoiconstituents).Use of the isolated phytoconstituents which are devoid of toxic effects of all other anticancer drugs and better absorption and cellular uptake due to its nano size..

Medienart:	E-Artikel

Erscheinungsjahr:	2021
Erschienen:	2021

Enthalten in:	Zur Gesamtaufnahme - volume:32
Enthalten in:	Journal of materials science / Materials in medicine - 32(2021), 2 vom: Feb.

Sprache:	Englisch

Beteiligte Personen:	Shejawal, Kiran P. [VerfasserIn] Randive, Dheeraj S. [VerfasserIn] Bhinge, Somnath D. [VerfasserIn] Bhutkar, Mangesh A. [VerfasserIn] Todkar, Sachin S. [VerfasserIn] Mulla, Anjum S. [VerfasserIn] Jadhav, Namdeo R. [VerfasserIn]

Links:	Volltext [kostenfrei]

BKL:	44.09$jMedizintechnik 51.40$jWerkstoffe für bestimmte Anwendungsgebiete

Anmerkungen:	© The Author(s) 2021

doi:	10.1007/s10856-021-06489-8

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2123596000