Details der Publikation - A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

Abstract A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase (ACP). The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol (PEG) modified indium-tin oxide (ITO) slide. In the presence of ACP, the peptide substrate was dephosphorylated under acidic condition to generate a new mass signal. The quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide substrate. Under optimal detection conditions, the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit (LOD) of 0.04 g/L. The designed ACP sensing chip has been used to analyze ACP in complex clinical samples, which exhibited high selectivity, good repeatability, and admirably anti-interference ability. This work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis, and provides a convenient method for the quantitation of proteases in clinical diagnosis..

Medienart:	E-Artikel

Erscheinungsjahr:	2020
Erschienen:	2020

Enthalten in:	Zur Gesamtaufnahme - volume:64
Enthalten in:	Science China / Chemistry - 64(2020), 1 vom: 28. Sept., Seite 151-156

Sprache:	Englisch

Beteiligte Personen:	Ma, Qiulin [VerfasserIn] Chen, Yunlong [VerfasserIn] Feng, Nan [VerfasserIn] Yan, Feng [VerfasserIn] Ju, Huangxian [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Anmerkungen:	© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020

doi:	10.1007/s11426-020-9850-3

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2122718536

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520			\|a Abstract A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase (ACP). The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol (PEG) modified indium-tin oxide (ITO) slide. In the presence of ACP, the peptide substrate was dephosphorylated under acidic condition to generate a new mass signal. The quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide substrate. Under optimal detection conditions, the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit (LOD) of 0.04 g/L. The designed ACP sensing chip has been used to analyze ACP in complex clinical samples, which exhibited high selectivity, good repeatability, and admirably anti-interference ability. This work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis, and provides a convenient method for the quantitation of proteases in clinical diagnosis.
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A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

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