Details der Publikation - Short-chain dehydrogenase/reductase, PsDeHase, from opium poppy: putative involvement in papaverine biosynthesis

Short-chain dehydrogenase/reductase, PsDeHase, from opium poppy: putative involvement in papaverine biosynthesis

Abstract Benzylisoquinoline alkaloids (BIAs) are medicinal compounds that are the vital constituents of several pharmaceutical preparations, including vasodilator with papaverine. Though intermediate steps and associated enzymes involved in the biosynthesis of key BIAs of opium poppy have been characterized, very limited information is available for the biosynthesis of papaverine. Through various studies, two metabolic routes utilizing (S)-norcoclaurine as substrate for the biosynthesis of papaverine have been suggested. These two controversial pathways for papaverine biosynthesis (NH and $ NCH_{3} $) include a short-chain dehydrogenase/reductase (SDR) that catalyzes dehydrogenation of tetrahydropapaverine to papaverine. In this work, we have identified and functionally characterized one SDR, PsDeHase, which might participate in the dehydrogenation in the papaverine biosynthesis. The expression analysis and metabolite profiling suggested a correlation between transcript and papaverine content. The PsDeHase expressed abundantly in stem and shared homology with reductases involved in the secondary metabolism. In silico investigation predicted tetrahydropapaverine as the possible substrate that formed a stable complex with PsDeHase. Repression of PsDeHase transcripts in opium poppy plants exposed to VIGS treatment led to a comparable decrease in the accumulation of papaverine..

Key message Using in silico and Virus-Induced Gene Silencing approaches, we demonstrate that PsDeHase, a Short-chain dehydrogenase/reductase, is putatively involved in papaverine biosynthesis in opium poppy..

Medienart:	E-Artikel

Erscheinungsjahr:	2020
Erschienen:	2020

Enthalten in:	Zur Gesamtaufnahme - volume:143
Enthalten in:	Plant cell, tissue and organ culture - 143(2020), 2 vom: 16. Sept., Seite 431-440

Sprache:	Englisch

Beteiligte Personen:	Agarwal, Parul [VerfasserIn] Pathak, Sumya [VerfasserIn] Kumar, Ravi Shankar [VerfasserIn] Dhar, Yogeshwar Vikram [VerfasserIn] Shukla, Sudhir [VerfasserIn] Asif, Mehar Hasan [VerfasserIn] Trivedi, Prabodh Kumar [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	42.03$jMethoden und Techniken der Biologie 42.40$jPflanzencytologie$jPflanzenhistologie$jPflanzenmorphologie 48.03$jMethoden und Techniken der Land- und Forstwirtschaft 48.56$jPflanzenvermehrung
Themen:	Benzylisoquinoline alkaloids Dehydrogenase NH pathway Opium poppy Papaverine biosynthesis

Anmerkungen:	© Springer Nature B.V. 2020

doi:	10.1007/s11240-020-01930-2

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2120562326

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520			\|a Abstract Benzylisoquinoline alkaloids (BIAs) are medicinal compounds that are the vital constituents of several pharmaceutical preparations, including vasodilator with papaverine. Though intermediate steps and associated enzymes involved in the biosynthesis of key BIAs of opium poppy have been characterized, very limited information is available for the biosynthesis of papaverine. Through various studies, two metabolic routes utilizing (S)-norcoclaurine as substrate for the biosynthesis of papaverine have been suggested. These two controversial pathways for papaverine biosynthesis (NH and $ NCH_{3} $) include a short-chain dehydrogenase/reductase (SDR) that catalyzes dehydrogenation of tetrahydropapaverine to papaverine. In this work, we have identified and functionally characterized one SDR, PsDeHase, which might participate in the dehydrogenation in the papaverine biosynthesis. The expression analysis and metabolite profiling suggested a correlation between transcript and papaverine content. The PsDeHase expressed abundantly in stem and shared homology with reductases involved in the secondary metabolism. In silico investigation predicted tetrahydropapaverine as the possible substrate that formed a stable complex with PsDeHase. Repression of PsDeHase transcripts in opium poppy plants exposed to VIGS treatment led to a comparable decrease in the accumulation of papaverine.
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Short-chain dehydrogenase/reductase, PsDeHase, from opium poppy: putative involvement in papaverine biosynthesis

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