Physiological and transcriptional response to heat stress in heat-resistant and heat-sensitive maize (Zea mays L.) inbred lines at seedling stage
Abstract To understand the molecular and physiological mechanism underlying the heat stress in maize, transcriptional and physiological response to heat stress in the heat-resistant Huangzaosi (HZS) and heat-sensitive Lv-9-Kuan (L9K) inbred lines at seedling stage were analyzed and compared at seedling stage. Our results indicated that MDA content of the two inbred lines increased significantly under heat stress; the values of MDA in L9K was significantly higher than that in HZS. The level of SOD, CAT, and POD enzyme activities in HZS was higher than those in L9K for both the heat-treated group and controls. The values of Fv/Fm, qP, and ФPSII reduced by heat stress in L9K were higher than the respective values in HZS. RNA-seq data showed that heat stress induced more heat stress-related genes in HZS (257 heat stress-related genes) than in L9K (224 heat stress-related genes). GO and KEGG enrichment analyses indicated that HZS and L9K changed their physiological and biochemical mechanisms in response to heat stress through different molecular mechanisms. Weighted Gene Co-expression Network Analysis showed that HZS might obtain stronger heat resistance than L9K through a unique transcriptional regulatory network. Our findings provide insights into the molecular networks that mediate the tolerance of maize heat stress and also help us to mine key heat stress-related genes..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:257 |
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| Enthalten in: |
Protoplasma - 257(2020), 6 vom: 29. Juli, Seite 1615-1637 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, De-Chuan [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Differentially expressed genes (DEGs) |
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| Anmerkungen: |
© Springer-Verlag GmbH Austria, part of Springer Nature 2020 |
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| doi: |
10.1007/s00709-020-01538-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2120088608 |
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| 245 | 1 | 0 | |a Physiological and transcriptional response to heat stress in heat-resistant and heat-sensitive maize (Zea mays L.) inbred lines at seedling stage |
| 264 | 1 | |c 2020 | |
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| 500 | |a © Springer-Verlag GmbH Austria, part of Springer Nature 2020 | ||
| 520 | |a Abstract To understand the molecular and physiological mechanism underlying the heat stress in maize, transcriptional and physiological response to heat stress in the heat-resistant Huangzaosi (HZS) and heat-sensitive Lv-9-Kuan (L9K) inbred lines at seedling stage were analyzed and compared at seedling stage. Our results indicated that MDA content of the two inbred lines increased significantly under heat stress; the values of MDA in L9K was significantly higher than that in HZS. The level of SOD, CAT, and POD enzyme activities in HZS was higher than those in L9K for both the heat-treated group and controls. The values of Fv/Fm, qP, and ФPSII reduced by heat stress in L9K were higher than the respective values in HZS. RNA-seq data showed that heat stress induced more heat stress-related genes in HZS (257 heat stress-related genes) than in L9K (224 heat stress-related genes). GO and KEGG enrichment analyses indicated that HZS and L9K changed their physiological and biochemical mechanisms in response to heat stress through different molecular mechanisms. Weighted Gene Co-expression Network Analysis showed that HZS might obtain stronger heat resistance than L9K through a unique transcriptional regulatory network. Our findings provide insights into the molecular networks that mediate the tolerance of maize heat stress and also help us to mine key heat stress-related genes. | ||
| 650 | 4 | |a Differentially expressed genes (DEGs) | |
| 650 | 4 | |a Heat stress | |
| 650 | 4 | |a qRT-PCR | |
| 650 | 4 | |a RNA sequencing (RNA-seq) | |
| 650 | 4 | |a Transcriptome | |
| 650 | 4 | |a Network analysis | |
| 700 | 1 | |a Zhu, Jia-Fei |4 aut | |
| 700 | 1 | |a Shu, Zhong-Ze |4 aut | |
| 700 | 1 | |a Wang, Wei |4 aut | |
| 700 | 1 | |a Yan, Cheng |4 aut | |
| 700 | 1 | |a Xu, Shan-Bin |4 aut | |
| 700 | 1 | |a Wu, De-Xiang |4 aut | |
| 700 | 1 | |a Wang, Cheng-Yu |4 aut | |
| 700 | 1 | |a Dong, Zhao-rong |4 aut | |
| 700 | 1 | |a Sun, Genlou |0 (orcid)0000-0001-9144-6072 |4 aut | |
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